Sequences from confirmation amplicon


Confirmation sequences for line SALK_085292c and hit At1g76090 are shown.
Line ID SALK_085292c
Locus At1g76090 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:28551658
Primers used for PCR 61cs - LBb3, show primer details
   
Sequence Name 10-K109155-SALK_085292C-61cs
GenBank Accession LN484544 [GenBank]
Sequencing primer gene specific primer, 61cs: 5'-CAATAGCAGAAAGAATCACAACCA-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K109155-SALK_085292C-61cs
GCATCCTTCCCATCTTTACCGGTTCCACCACGGTTTAGACGGTGGTTTAGCCAAATCTTT
CTCCTTCACTACCTCAAACCCAACTTTCTTCGCCGTCACGGCTATATCAGCGTAGCTTCT
TAGTCCAGGAAGTGCGTCTCCTCTCTCGATCCCTTGAATCACGTCCTTGTGTTCTTCGTC
ATCGTCTCTGTATTTTTCAGTGGTGACCCATTCGTAGGACACGAACAAAGATCCTGGTTT
CATCACTCTGAAGATCTCCGAGTATACTTCTTCGAGCTTAGGAGCGTGACACGTAGCTTC
GATCGAGTAAGCTCCGTCAAACGTGTTTTCATCGAACGGCATCTTTAAAAAGTTACCACA
AACGACGTTGCAGAGAGAATCAAGTCCAGCTTTCTTGTTGTGAAGCTTGGCTCGTTGCAC
TTGGTACTCGTTGATAGTGATTCCAGTGACTTGGGCCTTGGAATGGGCCGCGATGGCTCT
CATCGGCCCACCCACGCCGCAACCAGCGTCAAGAATCTTTTGTCCCGGTTTCACTTTGAT
GAGATCGACGGCCATTTCTTCGTGGATTCTTGTGGCGTCTTTGTCGGATTTTCCAGGGAC
ATGAGGAGAGAAATGGAAAGATTGTCCCCATCCCCACTCGTAG
   
Sequence Name 02-K109154-SALK_085292C-R210
GenBank Accession LN484300 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K109154-SALK_085292C-R210
CATTTGTTACCCGTCTCTCTACTGCACCGCTGGTCTCATTGCCGGCGCCGTCTACTGGTT
CATATGCGTCCTAGGTCCAGCAGAACGAAAAGGCAAACGAGCCTCTGATCTCTCCGGCGG
CTCAATCTCCGCAGAAAAAGTCAAAGACAACTATAACCAATACTGGTCTTTCTTCCGCAA
ACCAAAAGAGATCGAATCAGCCGAGAAAGTACCTGACTTCGTCGACACGTTCTACAATCT
TGTCACTGATATCTACGAGTGGGGATGGGGACAATCTTTCCATTTCTCTCCTCATGTCCC
TGGAAAATCCGACAAAGACGCCACAAGAATCCACGAAGAAATGGCCGTCGATCTCATCAA
AGTGAAACCGGGACAAAAGATTCTTGACGCTGGTTGCGGCGTGGGTGGGCCGATGAGAGC
CATCGCGGCCCATTCCAAGGCCCAAGTCACTGGAATCACTATCAACGAGTACCAAGTGCA
ACGAGCCAAGCTTCACAACAAGAAAGCTGGACTTGATTCTCTCTGCAACGTCGTTTGTGG
TAACTTTTTAAAGATGCCGTTCGATGAAAACACGTTTGACGGAGCTTACTCGATCGAAGC
TACGTGTCACGCTCCTAAGCTCGAAGAAGTATACTCGGAGATCTTCAGAGTGATGAAACC
AGGATCTTTGTTCGTGTCCTACGAATGGGTCACCACTGAAAAATA

Last Updated on 06.12.2018 16:11