Sequences from confirmation amplicon


Confirmation sequences for line SALK_085672c and hit At1g17430 are shown.
Line ID SALK_085672c
Locus At1g17430 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:5983022
Primers used for PCR 50vi - LBb3, show primer details
   
Sequence Name 52-K117945-SALK_085672C-50vi
GenBank Accession LN485566 [GenBank]
Sequencing primer gene specific primer, 50vi: 5'-AAGTGAAAGCAAACAAACGAGAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K117945-SALK_085672C-50vi
ATACTTAAACCCATAAAAACAATCCAAAACATCAATTAGTTCTCTATCAGCGACACATTA
ATCATCACGCCAACTTATTAATATATATCTTGATGCTTAGAGTTAAACCATTAGTGATCA
GGAATTAAAAAAAGGTTAACTCAACGAAATTTTATACTACCTGGGATATTACAGGCAATT
CTGGTTCCTCCTCTCGTTCCAACAAATTCTTAGCTAGCTCAAGAAGCTCCTGCCGATTTT
TCTCATACATTACCTATAAGAAGAGGATGAATTTTGCAAATTCCAAACCTTTAGGATCAT
GACTAACGAACAAACAATTTACGGGACCAGATTCGGAAATAAACAGATAAAACAGAAACA
GAACAAACTTACAGCGATGAATTGGGGTGTAAACAAATTGACGCTTAGACAACTTAATAA
CACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCA
ACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGG
TTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCG
   
Sequence Name 17-K117944-SALK_085672C-R210
GenBank Accession LN484761 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K117944-SALK_085672C-R210
GTCATTTGTTACCCCCAATTCATCGCTGTAAGTTTGTTCTGTTTCTGTTTTATCTGTTTA
TTTCCGAATCTGGTCCCGTAAATTGTTTGTTCGTTAGTCATGATCCTAAAGGTTTGGAAT
TTGCAAAATTCATCCTCTTCTTATAGGTAATGTATGAGAAAAATCGGCAGGAGCTTCTTG
AGCTAGCTAAGAATTTGTTGGAACGAGAGGAGGAACCAGAATTGCCTGTAATATCCCAGG
TAGTATAAAATTTCGTTGAGTTAACCTTTTTTTAATTCCTGATCACTAATGGTTTAACTC
TAAGCATCAAGATATATATTAATAAGTTGGCGTGATGATTAATGTGTCGCTGATAGAGAA
CTAATTGATGTTTTGGATTGTTTTTATGGGTTTTAAGTATTCATCTTTTGGATAGTGGAA
ACACTATTCTCGTTTGTTTGNTTTCACTT

Last Updated on 06.12.2018 16:11