Sequences from confirmation amplicon


Confirmation sequences for line SALK_085849c and hit At3g22970 are shown.
Line ID SALK_085849c
Locus At3g22970 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:8152864
Primers used for PCR 51vi - LBb3, show primer details
   
Sequence Name 46-K117945-SALK_085849C-51vi
GenBank Accession LN485422 [GenBank]
Sequencing primer gene specific primer, 51vi: 5'-TCATTGACGATCTTCTTCATATCG-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K117945-SALK_085849C-51vi
ATCTTCGCCGCGTCGGCTAACAGATTCCTCTCTCCGACGGTTGTGCACGGAATCAAACTC
TGTTTCAGATCGACAAATTCAAATTCAGTTTCATGTTTAAATCAAGAGAGATTAAGAATT
TGTTGTTGTTGTTGTTGTGGTTACCTTGAGATGATCAGAAGCGTCGCAACCGTCGATTGA
ACCACCGAATAGATCTGATTCATCATCGGAAGAACCATCGTTGTTGCCGTTAAAGCAATT
ACAACGATTACGTCCACATTTAGCTTGTTTCTCGTTGTAAACAAATATACGCTTGCGATT
ACGTCCACAATTTGTCACAGGATATATTGTGGTGTAAACAAATTGACGCTTAGACAACTT
AATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGAC
GGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCAC
GCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCGAATCGNGCAAAAT
   
Sequence Name 11-K117944-SALK_085849C-R210
GenBank Accession LN484596 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K117944-SALK_085849C-R210
GTCATTTGTTTACCACAATATATCCTGTGACAATTGTGGACGTAATCGCAAGCGTATATT
TGTTTCAACGAGAAACAAGCTAAATGTGGACGTAATCGTTGTAATTGCTTTAACGGCAAC
AACGATGGTTCTTCCGATGATGAATCAGATCTATTCGGTGGTTCAATCGACGGTTGCGAC
GCTTCTGATCATCTCAAGGTAACCACAACAACAACAACAACAAATTCTTAATCTCTCTTG
ATTTAAACATGAAACTGAATTTGAATTTGTCGATCTGAAACAGAGTTTGATTCCGTGCAC
AACCGTCGGAGAGAGGAATCTGTTAGCCGACGCGGCGAAGATTGTAGATAAGAACAAATC
GGTGAAACGAAAAGACGATATGAAGAAGATCGTCAATG

Last Updated on 06.12.2018 16:11