Sequences from confirmation amplicon


Confirmation sequences for line SALK_086199c and hit At5g06560 are shown.
Line ID SALK_086199c
Locus At5g06560 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:2003757
Primers used for PCR 13gq - LBb3, show primer details
   
Sequence Name 74-K112564-SALK_086199C-13gq
GenBank Accession LN486103 [GenBank]
Sequencing primer gene specific primer, 13gq: 5'-TGATGGAGCTTTAGTCTTTTGGAT-3'
Sequence (A. th genome BLAST matches underlined) 
>74-K112564-SALK_086199C-13gq
ATTCTTGCACTTTGGCATGTTTGACGGAGATCAGCCTTAGCGGCGAGGGTTTTGGGTTTT
ATCAGTTCACCACGGTTTCATTATTTAACCAGAAGATGGAATTGAGAAAATCTTGGTTCG
AACCGGTTCAACCGTAATAACTACTCAAGTACTGTATTACAGAATGTTGCTAATGTAGAA
TCATCATTGATTGATTACACATTTTGATTAATGCCTTTTAAAATTTGACAAAAATGATCT
ATTGATCGGTGATAGCCTGATAGGTTATAGTAAAATATTGAGAATCTGAGGATCAAGACA
ATGCCAAGTTCCATTAACCGGAAGTTCTGAGAGAGATAACCTTTTTTTTGTTTGTTTTCT
TTCCAGAGAAATTCAAAGAAGAATCTGGAAACATTATACACAGCTTACAATAGAAAGAAC
AAGACGTGCTTTTAGTGTAGCCTCACCGAGACAAATCATCAAACCCCATTCCAACCCAAC
AACAAATGACATATGGCAAATACACCAAGCAAAGAAAGATAAAAACTGCACATTAGAGAA
AAATCATTTTTCTCAGTAAAAACCATGAAGATGGAGAAAGTAAGTACTTTCACAAAGTAA
TTCACATTATGCGCTGCAAACCAAATTTCACACTTGTGTGCTTGAGAGACATCTCCATTG
CCGTATTCGAGGAGTCTTTTCTAGAAGCATTTGCAATCCCATGTTGTGGTGTAAACAAAT
TGACGCTNAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTT
CTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCNGGCCCTGAGAGAGT
TGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTT
C
   
Sequence Name 58-K112563-SALK_086199C-R210
GenBank Accession LN485689 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>58-K112563-SALK_086199C-R210
CATTTGTTACCCACAACATGGGATTGCAAATGCTTCTAGAAAAGACTCCTCGAATACGGC
AATGGAGATGTCTCTCAAGCACACAAGTGTGAAATTTGGTTTGCAGCGCATAATGTGAAT
TACTTTGTGAAAGTACTTACTTTCTCCATCTTCATGGTTTTTACTGAGAAAAATGATTTT
TCTCTAATGTGCAGTTTTTATCTTTCTTTGCTTGGTGTATTTGCCATATGTCATTTGTTG
TTGGGTTGGAATGGGGTTTGATGATTTGTCTCGGTGAGGCTACACTAAAAGCACGTCTTG
TTCTTTCTATTGTAAGCTGTGTATAATGTTTCCAGATTCTTCTTTGAATTTCTCTGGAAA
GAAAACAAACAAAAAAAAGGTTATCTCTCTCAGAACTTCCGGTTAATGGAACTTGGCATT
GTCTTGATCCTCAGATTCTCAATATTTTACTATAACCTATCAGGCTATCACCGATCAATA
GATCATTTTTGTCAAATTTTAAAAGGCATTAATCAAAATGTGTAATCAATCAATGATGAT
TCTACATTAGCAACATTCTGTAATACAGTACTTGAGTAGTTATTACGGTTGAACCGGTTC
GAACCAAGATTTTCTCAATTCCATCTTCTGGTTAAATAATGAAACCGTGGTGAACTGATA
AAACCCAAAACCCTCGCCGCTAAGGCTGATCTCCGTCAAACATGCCAAAGTGCAAGAATC
TGTTGCTAACCTCGAAGCGACGTANATCCAAATCCAAAAGACTAAAGNCTCCATC

Last Updated on 06.12.2018 16:11