Sequences from confirmation amplicon


Confirmation sequences for line SALK_086372 and hit At1g23760 are shown.
Line ID SALK_086372
Locus At1g23760 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:8403911
Primers used for PCR AW55 - R204, show primer details
   
Sequence Name 52-K106282-SALK_086372C-R210
GenBank Accession LN485546 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K106282-SALK_086372C-R210
GCGTCATTTGTTACTACTGTCACTCTGTGCCTAAGTTCGGGTGTACGAGTCAGATCTTTT
GGATCCCAAGAGTAAAGCCAAGATCAACCATGGTATTGCTATATGTCACATGGACACATC
AGCTTGGGGTGCGAACCATGGAGCCTTCATGTTGCTTGGGTCCCGGCCAGGTCAGATCGA
AGTATGCCATTGGATCTTTGAGAATGATATGAATTGGGCCATCGCCGATTAACCATGTTG
TAGCAAAATGAATAATGAATGTTAATGTTTTGTATCTCTATATTTCAGTAACGATACAAA
CTTACTATCTTCCTCATATTCTGTTAACTAAATATGTTGTATCAGGATTATGTAAACATT
CTAAAGCAATGAAGTTAAAAAAAAATAGCAGTGGAATGTAAATTTTCTAAAAATTATTGA
ATTCACCTAGACAATTTAAGTTGTGGGGGTTTAAGCAAACCGGTTCTACGAGAATCAGTC
CGGTCCAACTGATCATATCATCCAAGTTACGGTGACCATGTTTCTTTGTCTGGAAGTGTA
AGACTGATTTTACTGAACACAGTTCCAACATTCAGGGAAATGTTATTGATACAAGAATTT
CTCAATGAAGTAAAGGAGAGTGGATGC
   
Sequence Name 68-K106283-SALK_086372C-AW55
GenBank Accession LN485944 [GenBank]
Sequencing primer gene specific primer, AW55: 5'-TTGCATCACTTCTCCTTTACTTCA-3'
Sequence (A. th genome BLAST matches underlined) 
>68-K106283-SALK_086372C-AW55
AAATGCAGCAGCGGTCCACGCAAGACTGTGTTCAGTAAAATCAGTCTTACACTTCCAGAC
AAAGAAACATGGTCACCGTAACTTGGATGATATGATCAGTTGGACCGGACTGATTCTCGT
AGAACCGGTTTGCTTAAACCCCCACAACTTAAATTGTCTAGGTGAATTCAATAATTTTTA
GAAAATTTACATTCCACTGCTATTTTTTTTTAACTTCATTGCTTTAGAATGTTTACATAA
TCCTGATACAACATATTTAGTTAACAGAATATGAGGAAGATAGTAAGTTTGTATCGTTAC
TGAAATATAGAGATACAAAACATTAACATTCATTATTCATTTTGCTACAACATGGTTAAT
CGGCGATGGCCCAATTCATATCATTCTCAAAGATCCAATGGCATACTTCGATCTGACCTG
GCCGGGACCCAAGCAACATGAAGGCTCCATGGTTCGCACCCCAAGCTGATGTGTCCATGT
GACATATAGCAATACCATGGTTGATCTTGGCTTTACTCTTGGGATCCAAAAGATCTGACT
CGTACACCCGAACTTTAGGCACAGAGTGACAGTAGTAAACAAATTGACGCTTAGACAACT
TAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGA
CGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGC
Primers used for PCR AW56 - LBb3, show primer details
   
Sequence Name 61-K119294-SALK_086372C-AW56
GenBank Accession LN485780 [GenBank]
Sequencing primer gene specific primer, AW56: 5'-GACATTAAGGATAAAATGCCGAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>61-K119294-SALK_086372C-AW56
CTCGACTCCGTTTTCTCATCGAAATCGCGGAAATCAGCGTGTTTTCCACGCTAACGATAA
CTCGACGATGGAAGGGATAATTACTGATGCGGTAAGGGAATGTGAGAGACCGCCGACAGT
CAGCGAGACTAAACGATGTGTCGGATCAGCGGAGGATATGATCGATTTCGCGACATCTGT
GCTTGGTCGAAGTGTGGTACTCCGGACGACAGAGAGTGTAGCCGGATCAAAGGAGAAAGT
CATGATCGGTAAAGTCAATGGAATCAACGGTGGAAGAGTGACAAAATCAGTGTCGTGTCA
CCAAAGTTTGTGGTGTAAACAAATTGACGCTTAGACAACTTAATAACACATTGCGGACGT
TTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCC
TTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGG
CGAAAATCCTGTTTGATGGTGATTCCGAAATCGGCAAAA
   
Sequence Name 13-K119293-SALK_086372C-R210
GenBank Accession LN484657 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K119293-SALK_086372C-R210
GTCATTTGTTACCCACAAACTTTGGTGACACGACACTGATTTTGTCACTCTTCCACCGTT
GATTCCATTGACTTTACCGATCATGACTTTCTCCTTTGATCCGGCTACACTCTCTGTCGT
CCGGAGTACCACACTTCGACCAAGCACAGATGTCGCGAAATCGATCATATCCTCCGCTGA
TCCGACACATCGTTTAGTCTCGCTGACTGTCGGCGGTCTCTCACATTCCCTTACCGCATC
AGTAATTATCCCTTCCATCGTCGAGTTATCGTTAGCGTGGAAAACACGCTTGATTTCCGC
GATTTTCGATGTAGAAAACGGTAGTTTCGAGACAATGCTACGAGGTAGAAACGACCTTTT
CGGCATTTTATCCTTAATGT

Last Updated on 06.12.2018 16:11