Sequences from confirmation amplicon


Confirmation sequences for line SALK_086656c and hit At5g67340 are shown.
Line ID SALK_086656c
Locus At5g67340 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:26865680
Primers used for PCR 25ht - LBb3, show primer details
   
Sequence Name 82-K112669-SALK_086656C-25ht
GenBank Accession LN486245 [GenBank]
Sequencing primer gene specific primer, 25ht: 5'-TCTTTTTCGTTTTCTTTGTTCGAT-3'
Sequence (A. th genome BLAST matches underlined) 
>82-K112669-SALK_086656C-25ht
GCATCAGATTCTGTCCTCAGCTTTATGATATTGGTTACAATTATATTCGTTGAATTTTCA
GCTTAGCTTTGAATGTCCTAAACTTGTTCTTACTTGGTGTTCTTGCAGTTCTTGTTTCAG
GGGTTTTGTATATTTGCCTCAACTTTTCATTGATTCACGAATTTATTTCATTACATGATG
GTAAGTTTCTAAATGTCTATGCCCTCAAATAAACGAGGTTTGATTTTGTTTTTCTTACTA
ATTTATAAACTCATGTTGTTGTTTTGGTAAGTGAAGGTACATATGGAGGTGTCTTGGTTA
AGAGTTCTTCTAGATAACATCTCCTCCTATCTAAGTTTATCATCTATGGACGATTTATCT
TCAAACCCTGCTCATAAGTACTACACCAGAGGAGAAGATATAGGAAAGCTTATCAAGCCT
GTTCTTGAGAACCTCATTGACTCTGACGCGGCTCCTAGCGAGTTGCTTAACAATGGTTTT
GAAGAATTAGCTCAATACGTTGATGAACTTAGAGAACAGTTTCAGAGTTGGCAACCTCTT
TCAACTAGAATCTTTTATGTAAGTATGCAAAAAACAGAATTTACATCTTCATTTATAGCT
GTTTCTGAGCTAACTCTTGTCTGTTTTCAGGTTCTTCGAATTGAATCATTAGCATCAAAG
TTACGAGAATCCAGTTTGGAAGTCTTTCAGCTCCTCAAACACTGCGAACAACATTTGCCT
GCTGACTTGATCTCACCTTCTTTTGAGGTAAACTCAATGATCTGTCTCTGTGTATAGTTT
TCTTTAGGATCTTATTATTGATCAGGCTCCTTGTTGCATATNGCANGAGTGCATTGAATT
GGTGAAGTTAGTGGCAAGAAAGCAAATATCACATTGC
   
Sequence Name 72-K112668-SALK_086656C-R210
GenBank Accession LN486057 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>72-K112668-SALK_086656C-R210
CATTTGTCGCATGTGATATTTGCTTTCTTGCCACTAACTTCACCAATTCAATGCACTCCT
GCAATATGCAACAAGGAGCCTGATCAATAATAAGATCCTAAAGAAAACTATACACAGAGA
CAGATCATTGAGTTTACCTCAAAAGAAGGTGAGATCAAGTCAGCAGGCAAATGTTGTTCG
CAGTGTTTGAGGAGCTGAAAGACTTCCAAACTGGATTCTCGTAACTTTGATGCTAATGAT
TCAATTCGAAGAACCTGAAAACAGACAAGAGTTAGCTCAGAAACAGCTATAAATGAAGAT
GTAAATTCTGTTTTTTGCATACTTACATAAAAGATTCTAGTTGAAAGAGGTTGCCAACTC
TGAAACTGTTCTCTAAGTTCATCAACGTATTGAGCTAATTCTTCAAAACCATTGTTAAGC
AACTCGCTAGGAGCCGCGTCAGAGTCAATGAGGTTCTCAAGAACAGGCTTGATAAGCTTT
CCTATATCTTCTCCTCTGGTGTAGTACTTATGAGCAGGGTTTGAAGATAAATCGTCCATA
GATGATAAACTTAGATAGGAGGAGATGTTATCTAGAAGAACTCTTAACCAAGACACCTCC
ATATGTACCTTCACTTACCAAAACAACAACATGAGTTTATAAATTAGTAAGAAAAACAAA
ATCAAACCTCGTTTATTTGAGGGCATAGACATTTAGAAACTTACCATCATGTAATGAAAT
AAATTCGTGAATCAATGAAAAGTTGAGGCAAATATACAAAACCCCTGAAACAAGAACTGC
AAGAACACCAAGTAAGAACAAGTTTAGGACATTCAAAGCTAAGCTGAAAATTCAACGAAT
ATAATTGTAACCAATATCATAAAGCTGAGGACAGAATCTGATGCTTAGTCAACCCTCGGA
GTCACATAATAAAACCCCAATAAAAAAAACTCTGAATCGAACAA

Last Updated on 06.12.2018 16:11