Sequences from confirmation amplicon


Confirmation sequences for line SALK_086996c and hit At3g05190 are shown.
Line ID SALK_086996c
Locus At3g05190 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:1473825
Primers used for PCR 34fr - LBb3, show primer details
   
Sequence Name 55-K111957-SALK_086996C-34fr
GenBank Accession LN485624 [GenBank]
Sequencing primer gene specific primer, 34fr: 5'-AGGGTTACTTACAATGTTGGTTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K111957-SALK_086996C-34fr
GCATATGGCGTCAGCAGCATTCGTGTTGTTACTTTCAATCTGTTTGTTCCATTTGCAGAG
AAAATTAATCTCATAGTAAAAACACAATGTCAATTTCTTGAACTCTCTTATTTTGAATAC
AGTACCTTTGCTAGTATGTTATTGAGAAGGTTGTTGTGATGAATCTTGGAATCCAAATTC
TGACAAAACAAAAAGAATTTTAGAAACCAACAATTTTACAGACAAGGATTTTTTATGTTG
TAACATGAAGAGAATGTGGAACCAAATTCATACATTAGGCGAATTGCGGCGTGTGGTTGC
AGTCACCAGCACAATTCCACCTTCATTGTCATATACCGGAGGCTTCCATTCGGCAAGCAC
TGCATTATTTGAAAGAAAGATATATTGTGGTGTAAACAAATTGACGCTTAGACAACTTAA
TAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGG
GCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGC
TGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCGA
   
Sequence Name 51-K111956-SALK_086996C-R210
GenBank Accession LN485535 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K111956-SALK_086996C-R210
CATTTGTTTCACCACAATATATCTTTCTTTCAATAATGCAGTGCTTGCCGAATGGAAGCC
TCCGGTATATGACAATGAAGGTGGAATTGTGCTGGTGACTGCAACCACACGCCGCAATTC
GCCTAATGTATGAATTTGGTTCCACATTCTCTTCATGTTACAACATAAAAAATCCTTGTC
TGTAAAATTGTTGGTTTCTAAAATTCTTTTTGTTTTGTCAGAATTTGGATTCCAAGATTC
ATCACAACAACCTTCTCAATAACATACTAGCAAAGGTACTGTATTCAAAATAAGAGAGTT
CAAGAAATTGACATTGTGTTTTTACTATGAGATTAATTTTCTCTGCAAATGGAACAAACA
GATTGAAAGTAACAACACGAATGCTGCTGACGCCATTATGCTTGACAAAGATGGTTATGT
GTCTGAAACCAATGCAACCAACATTGTAGNTAACCC

Last Updated on 06.12.2018 16:11