Sequences from confirmation amplicon


Confirmation sequences for line SALK_087083c and hit At2g15880 are shown.
Line ID SALK_087083c
Locus At2g15880 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:6919275
Primers used for PCR 40wt - R212, show primer details
   
Sequence Name 29-K118709-SALK_087083C-40wt
GenBank Accession LN485118 [GenBank]
Sequencing primer gene specific primer, 40wt: 5'-GAAAATGGTGAACTTCCTGATGAT-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K118709-SALK_087083C-40wt
CCCAGGGAAAAGGCGGTGTATTCAGACCCGTTTAACACCACAGGAAACTGGCATGGCCCT
CATGTGTGTGGCTATACGGGTGTGTTCTGTGCCCCGGCTCTTGATGATCCTGACGTCGCG
GTTGTGGCGGGGGTTGATTTGAATGGTGCGGATATCGCAGGGCATTTACCGGCTGAACTT
GGTTTGATGACAGATGTTGCGATGTTTCATTTGAATTCAAACCGGTTCTGTGGTATCATA
CCTAAGAGTTTCGAGAAGTTGAGCCTAATGCACGAGTTTGATGTCAGCAACAACCGGTTT
GTCGGACCTTTCCCTTCTGTTGTCCTCTCGTGGCCGGCTGTTAAGTTCATTGACGTCAGA
TACAATGATTTCGAAGGTCAAGTCCCTCCTGAGCTTTTCAAAAAGGATCTCGACGCCATT
TTCCTGAACAACAACAGATTCACGTCTACCATTCCTGATTCCCTCGGAGAATCATCAGCC
TCCGTTGTGACCTTTGCTCACAACAAATTTAGCGGTTGTATCCCTAGAAGTATTGGAAAC
ATGAAGAATCTCAACGAGATTATCTTTAAGGACAACAGTCTTGGCGGTTGTTTCCCGTCA
GAGATTGGGAAGCTAGCCAATGTGAACGTGTTTGACGCTAGCATGAACTCCTTCACTGGT
GTTCTACCTCCAAGCTTTGTCGGGCTTACCAGTATGGAAGAGTTTGATATCTCTGGGAAT
AAACTCACCGGGTTCAACACTGATAGTTTAAACTGAAGGCGGGAAACGACAATCTGATCA
TGAGCGGAGAATTAAAGGAGTCACGTTATGAC
   
Sequence Name 04-K118708-SALK_087083C-R214
GenBank Accession LN484386 [GenBank]
Sequencing primer gene specific primer, R214: 5'-AACGTTGCGGTTCTGTCAGTTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K118708-SALK_087083C-R214
CGGCGGGGGTCATAACGTGACTCCCTTAATTCTCCGCTCATGATCAGATTGTCGTTTCCC
GCCTTCAGTTTAAACTATCAGTGTTGAACCCGGTGAGTTTATTCCCAGAGATATCAAACT
CTTCCATACTGGTAAGCCCGACAAAGCTTGGAGGTAGAACACCAGTGAAGGAGTTCATGC
TAGCGTCAAACACGTTCACATTGGCTAGCTTCCCAATCTCTGACGGGAAACAACCGCCAA
GACTGTTGTCCTTAAAGATAATCTCGTTGAGATTCTTCATGTTTCCAATACTTCTAGGGA
TACAACCGCTAAATTTGTTGTGAGCAAAGGTCACAACGGAGGCTGATGATTCTCCGAGGG
AATCAGGAATGGTAGACGTGAATCTGTTGTTGTTCAGGAAAATGGCGTCGAGATCCTTTT
TGAAAAGCTCAGGAGGGACTTGACCTTCGAAATCATTGTATCTGACGTCAATGAACTTAA
CAGCCGGCCACGAGAGGACAACAGAAGGGAAAGGTCCGACAAACCGGTTGTTGCTGACAT
CAAACTCGTGCATTAGGCTCAACTTCTCGAAACTCTTAGGTATGATACCACAGAACCGGT
TTGAATTCAAATGAAACATCGCAACATCTGTCATCAAACCAAGTTCAGCCGGTAAATGCC
CTGCGATATCCGCACCATTCAAATCAACCCCCGCCACAACCGCGACGTCAGGATCATCAA
GAGCCGGGGCACAGAACACACCCGTATAGCCACACACATGAGGGCCATGCCAGTTTCCTG
TGGTGTTAAACGGGTCTGAATACACCGCCTTTTTCCAGGCTTGGAGAGCAATATACGCTC
TCTTAAGCCTATGGTTTGCAAATGTCACCTTAAGATCAACCTCATACTCAATATCATCAG
G
Primers used for PCR 13in - LBb3, show primer details
   
Sequence Name 82-K119948-SALK_087083C-R210
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>82-K119948-SALK_087083C-R210
TACCCGGTGAATATATGCAGTTGCCTAAATTGGTTAACTTGACGTACGCCTACAATTACT
TCAACGGACAAGGTGATTCATGTGTTCCAGGTAGTCAGAAGCAGATTGCATTGGATGATA
CACGCAACTGCTTGCCAGATCGGCCTAAGCAACGATCGGCCAAGGAATGTGCTGTGGTGA
TTAGCCGTCCAGTTGATTGTAGTAAGGACAAGTGTGCTGGTGGTTCTAGCCAAGCTACAC
CGTCGAAGTCTCCATCACCTGTGCCAACTAGGCCAGTTCATAAACCACAACCACCAAAAG
AATCACCACAACCAAATGACCCATATAATCAGTCTCCAGTGAAGTTTCGGCGTAGCCCGC
CTCCACCACAACAACCACATCACCATGTGGTTCACTCTCCACCGCCAGCTTCGTCTCCTC
CTACCTCACCACCAGTCCATTCAACTCCATCACCGGTTCACAAACCACAACCACCGAAAG
AGTCACCGCAACCAAATGATCCATATGATCAGTCACCTGTGAAGTTCCGGCGTAGTCCAC
CTCCACCTCCTGTTCACTCTCCCCCACCTCCGTCACCTATTCACTCACCACCACCACCAC
CAG

Last Updated on 06.12.2018 16:11