Sequences from confirmation amplicon


Confirmation sequences for line SALK_087356 and hit At4g17490 are shown.
Line ID SALK_087356
Locus At4g17490 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:9753047
Primers used for PCR CE31 - R204, show primer details
   
Sequence Name 57-K106282-SALK_087356C-R210
GenBank Accession LN485654 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K106282-SALK_087356C-R210
GTCATTTGTTACCCACAATATATCCTGGATATATCGCCGTTATCTCCACACCCACCGTTT
GGTTATCCACAATTGACCGTTGTTTGAATTCTATTTTTCTGTTTGGGTCAGCTTGTTGTA
TGCTGACGTGGAATTATGCTATATCTTTTGCTTATTTTATGTTCACATTCGAGCAAAATT
TATTCATTCGTTTATTTGTTTTTTAATACAACTCTTGATATTTCTAAATTCTAACCAATC
ACCTTATTTAGCGGCTACTGAGACAAAAATCGCATGTAAAGGCGAATCAATATGGAAAGA
ACAGAGAACACATAACACGAAGCTCCGATCAAATGACTTTTTATCATAGAACGTATTATT
TTTCAATGGCATGTAATATGCTCGGATTTATTACGNTAACAT
   
Sequence Name 73-K106283-SALK_087356C-CE31
GenBank Accession LN486070 [GenBank]
Sequencing primer gene specific primer, CE31: 5'-GATGTTACGGTAATAAATCCGAGC-3'
Sequence (A. th genome BLAST matches underlined) 
>73-K106283-SALK_087356C-CE31
GTTCATGATAAAAAGTCATTTGATCGGAGCTTCGTGTTATGTGTTCTCTGTTCTTTCCAT
ATTGATTCGCCTTTACATGCGATTTTTGTCTCAGTAGCCGCTAAATAAGGTGATTGGTTA
GAATTTAGAAATATCAAGAGTTGTATTAAAAAACAAATAAACGAATGAATAAATTTTGCT
CGAATGTGAACATAAAATAAGCAAAAGATATAGCATAATTCCACGTCAGCATACAACAAG
CTGACCCAAACAGAAAAATAGAATTCAAACAACGGTCAATTGTGGATAACCAAACGGTGG
GTGTGGAGATAACGGCGATATATCCAGGATATATTGTGGTGTAAACAAATTGACGCTTAG
ACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCA
GTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGC
GATCCACG
Primers used for PCR 80xo - LBb3, show primer details
   
Sequence Name 58-K119294-SALK_087356C-80xo
GenBank Accession LN485703 [GenBank]
Sequencing primer gene specific primer, 80xo: 5'-TAAATGTTTTTGGGCAGCTATACG-3'
Sequence (A. th genome BLAST matches underlined) 
>58-K119294-SALK_087356C-80xo
TTGGCGACATACGAAAACATCGTCGACATTCGACAAAGAGAATCTAGTCTTCATAACTAA
AATATTTTTAATCCAAAAACAAAAATAACATCCGACGTACGTGAAGTAAAACCAGTGTTT
TTCTTCCATCAAATTTTGCAACTATCATAAGTTTTTATATAAAAACGTGTGTTTATCCAA
ACATTGAGTTGATACGGCCGCTAAATTTTGATATTGCAAATGCATGCATGCGAAGAATTC
TCACAACGAGTAGTAAATTAATCAAATATTAACACGAAAGGTACTTTATAATAACTATTA
CTAAACCGTACTAATATTTTGATAAACATGAATCGATAAATTCTTGTCTAATCATCTGCC
AAAGAAACCCAATATTAGAGCGCAGCCTAATTAAAATATTTGTGTTTTGTTTTTTTACCC
CAGCTGCCATTGATGAACCACACGATCAAACCGTTGACGAGGGGTTTATAATCTAATCAA
ACGGTGTAAAACCGGTGAAACACGCGTGTACATACACCATCCCGCATTTGATAATTTTAG
ATTTTAAGTTTAAAAAATCCTGAGGATGTTGCCTTGAAAGAGTCTCTCATTGACTTGACT
CTTTCCTATAAATACACCTTTCCACTCTCTCTTCTCCACTGTCAAATCAAATCCCTCTTC
CTCTTCAATTCTCTGTTTCCTCCAAAATGGCTACACCAAACGAAGTATCAGCTCTTTTCC
TCATCAAGAAGTATCTCCTCGACGAATTGTCTCCGTTGCCTACTACTGCCACCACCAATC
GATGGATGAACGATTTCACGTCATTTGATCAAACCGGTTTCGAGTTTTCTGAATTTGAAA
CCAAACCGGAAATAATCGATCTCGTCACTCCCAAACCGGAGATTTTTGATTTCAATGTGA
AATCTGAAAATTCCATC
   
Sequence Name 10-K119293-SALK_087356C-R210
GenBank Accession LN484571 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K119293-SALK_087356C-R210
ACTCCGTCAATCCAGTCATCTATCGCCGTCAACCTGACTCAACATTCTCGCCGCCGCTAA
CGTCGTAACTCTCCTCCGTCTTTAGCACTTTCTCAACCACCGTCACTTCCTCCTCCTCGC
CGTCTCTCTTCCGTTTGTTGTACAGGCCACGACCATCTTCAGCGCGTGGATTCCACTTGT
CAACTTCAAGCGGGAAATTCAGAATCGCCTTTGATCCTCGTAGTCTAAACGCTTCTTTGT
CGTAAGCTCTAGCCGCTTCGATCGCCGTCTCAAACGTCCCGAGCCAAACACGAGTTCCAC
GACGAGTCGGGTCTCGAATCTCCGCCGCGAATTTCCCCCACGGCCTCATCCTCACTCCTC
TGTAATGCCTCTTCTCCTCTGCTGCTACAACCGGTACGGGAAGTTCCGGTTTAGGATTTC
CGGTTGCGAATTGAATCCACTTGGTTCGGTTCGGTGGTGCGATCTTCAACGGCGGTTTTC
GATTGGATTGAACAGTAACGCGAGGAGGATTCGATTGGAACGTGAAGGAATCGTTCGATT
CAGATGGAATTTCAGATTTCACATCGAAATCAAAAATCTCCGGTTTGGGAGTGACGAGAT
CGATTATTTCCGGTTTGGTTTCAAATTCAGAAAACTCGAAACCGGTTTGATCAAATGACG
TGAAATCGTTCATCCATCGATTGGTGGTGGCAGTAGTAGGCAACGGAGACAATTCGTCGA
GGAGATACTTCTTGATGAGGAAAAGAGCTGATACTTCGTTTGGTGTAGCCATTTTGGAGG
AAACAGAGAATTGAAGAGGAAGAGGGATTTGATTTGACAGTGGAGAAGAGAGAGTGGAAA
GGTGTAT

Last Updated on 06.12.2018 16:11