Sequences from confirmation amplicon


Confirmation sequences for line SALK_087377c and hit At5g08100 are shown.
Line ID SALK_087377c
Locus At5g08100 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:2594050
Primers used for PCR 02lv - LBb3, show primer details
   
Sequence Name 68-K113949-SALK_087377C-02lv
GenBank Accession LN485958 [GenBank]
Sequencing primer gene specific primer, 02lv: 5'-GTCTTTAAGTTATACGGCGGAAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>68-K113949-SALK_087377C-02lv
TCCGATCGATCTCCCCGACGAGCGACGTATCCCTCGTGAGAGCGCCCTCCGTCACTGCCT
CGATCTTGGCATCTCCGCCCTCAAATCCGGCAAGCCTCCCTTGGACGTCGCCGAACTTGT
CGTACGCCTCTCTTTCTCGATAATTGTTTTTTTTTCTTTGGCATATGGCAGCTGGTCGGA
TCCATGCGTTTTGATAATTTTGTCTAGAGTTTGGAATAATCGATGACGAAACAGAGAATT
CGGAACCAATTTTAGATTTATTTTTAAAACTCACTCTCTCTAGTGATCAGTTAACTAGTC
CCAAATTTGATACGATCACCATTTGTAAGGTTCGTGAACTTGAGAACCACCCGGACTTCA
ATGCGGGTAAAGGATCTGTCTTAACTGCACAAGGCACTGTTGAAATGGAAGCTTCCATTA
TGGACGGTAAAACCAAAAGATGTGGAGCTGTCTCCGGCTTGACCACTGTTGTTAATCCCA
TTTCTTTAGCTCGATGATAGTTTATTTATTAAACACTGATAGTTTAAACTATAAGCGTCA
AGTTGTCCAGGATATATTGTGGTGTAAACAAATTGACGCTTAGACAACTTAATAACACAT
TGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGC
TGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGC
CCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCC
   
Sequence Name 57-K113948-SALK_087377C-R210
GenBank Accession LN485667 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K113948-SALK_087377C-R210
ACCACAATATATCCTGGACAACTTGACGCTTATAGTTTAAACTATCAGTGTTTAATAAAT
AAACTATCATCGAGCTAAAGAAATGGGATTAACAACAGTGGTCAAGCCGGAGACAGCTCC
ACATCTTTTGGTTTTACCGTCCATAATGGAAGCTTCCATTTCAACAGTGCCTTGTGCAGT
TAAGACAGATCCTTTACCCGCATTGAAGTCCGGGTGGTTCTCAAGTTCACGAACCTTACA
AATGGTGATCGTATCAAATTTGGGACTAGTTAACTGATCACTAGAGAGAGTGAGTTTTAA
AAATAAATCTAAAATTGGTTCCGAATTCTCTGTTTCGTCATCGATTATTCCAAACTCTAG
ACAAAATTATCAAAACGCATGGATCCGACCAGCTGCCATATGCCAAAGAAAAAAAAACAA
TTATCGAGAAAGAGAGGCGTACGACAAGTTCGGCGACGTCCAAGGGAGGCTTGCCGGATT
TGAGGGCGGAGATGCCAAGATCGAGGCAGTGACGGAGGGCGCTCTCACGAGGGATACGTC
GCTCGTCGGGGAGATCGATCGGAATGTCTCCGGCACCGCCGTGTAGCGCAATCGCCCACC
CCACCATCTTCCGCCGT

Last Updated on 06.12.2018 16:11