Sequences from confirmation amplicon


Confirmation sequences for line SALK_087410c and hit At4g26620 are shown.
Line ID SALK_087410c
Locus At4g26620 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:13427928
Primers used for PCR CE41 - R204, show primer details
   
Sequence Name 74-K106742-SALK_087410C-R210
GenBank Accession LN486097 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>74-K106742-SALK_087410C-R210
CGTCATTTGTTTCCGAGCTGCAGAACCTAATGGTTGTTGCCACAAAATGGGAACAGCTCT
TCATGTTGTCAAGACGACACTACTCTGATGTTGTCACTGGGAACATCAGAAGATAATCAA
CTTGAAAGTGAAAACAACACTGAGAAGCTGACACCAGGAAGAAAAATTGCAGAAAAGACT
TTCTTTAGAATTAATAGTGACAAAGGATCTTCGACACGTAAGGTTTGCGGAATACCAACA
TGGTTAGAGAGCTGGGAGCGAGAAGATACTTATGCTGCTCTTGCTGTTGTCTGTGCTGCT
GCTTCCGTGGCTGTTGCCTATACTTGCTACAAACAGTTGTAACGAAGAAATTTGTAGCCC
TAAGATGATTGTGTATTGTGTTCTTATAAGACATTGTGATTTTGTTTCTGCTTTCAAACT
ACATTTTCCTTTCGATTTAAAGGAGGGTTCTTGTCAGAACTGTTTGATGATATGTATCCT
GTTAACTCTGCTTCTGCTTC
   
Sequence Name 90-K106743-SALK_087410C-CE41
GenBank Accession LN486331 [GenBank]
Sequencing primer gene specific primer, CE41: 5'-AGAAGCAGAAGCAGAGTTAACAGG-3'
Sequence (A. th genome BLAST matches underlined) 
>90-K106743-SALK_087410C-CE41
CTCCTTTAATCGAAAGGAAAATGTAGTTTGAAAGCAGAAACAAAATCACAATGTCTTATA
AGAACACAATACACAATCATCTTAGGGCTACAAATTTCTTCGTTACAACTGTTTGTAGCA
AGTATAGGCAACAGCCACGGAAGCAGCAGCACAGACAACAGCAAGAGCAGCATAAGTATC
TTCTCGCTCCCAGCTCTCTAACCATGTTGGTATTCCGCAAACCTTACGTGTCGAAGATCC
TTTGTCACTATTAATTCTAAAGAAAGTCTTTTCTGCAATTTTTCTTCCTGGTGTCAGCTT
CTCAGTGTTGTTTTCACTTTCAAGTTGATTATCTTCTGATGTTCCCAGTGACAACATCAG
AGTAGTGTCGTCTTGACAACATGAAGAGCTGTTCCCATTTTGTTGGCAACAACCATTAGG
TTCTGCAGCTCGGAAACAAATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTT
TAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTC
ACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACG
Primers used for PCR CE42 - LBb3, show primer details
   
Sequence Name 19-K119295-SALK_087410C-R210
GenBank Accession LN484838 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K119295-SALK_087410C-R210
CATTTGTTTACCCACAAATTTGTCAATTTGCTCTTTTCTCTTATGTGGTACAGGAAACAT
CGGCGTTGTGAACCGATGATTCTTGACTCACTTTCCCATTGATCTTTACGGAATGGACTG
TACCATTTAGCTGAAATCGCCCTTCTTGAGTTTTCTTCTGATCTTCTTCTGATAGACCCA
TTTCACCCCTAATAATCAGAGAAGCATCATGTGTGAATCTACCAATTACCACTAATCCGG
AAACTGCGAGAAAACTGACAGAACAAGAAAACTTTGTACGTACCTCCATAGCCGGTCTAC
AATCTCGCCTTTGTTGATGTGCTGCTCCAAAAGAATAGGTA

Last Updated on 06.12.2018 16:11