Sequences from confirmation amplicon


Confirmation sequences for line SALK_087519c and hit At3g49880 are shown.
Line ID SALK_087519c
Locus At3g49880 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:18496976
Primers used for PCR 15vt - LBb3, show primer details
   
Sequence Name 72-K118240-SALK_087519C-15vt
GenBank Accession LN486066 [GenBank]
Sequencing primer gene specific primer, 15vt: 5'-CTTCGTTTTTCCATGTCCATAAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>72-K118240-SALK_087519C-15vt
ATCCAAGAAGCTGTTTTCACATTCTAATTCTAAAGTCCAAGAGTGAGACATTAATGGAAA
GCAAGAAACGGTTAAAACTTACTCTAGCAGCACCTTTCTTGTGAGAAAGATAAGTTGGTC
CATCTTTATATTCACCATACCAATAGTAAACCTTTGATATGTCATCAAACAAAATGCCAC
CACCGTGTGCTTGAATCGGATTCCCTTCCGTGTCTGTCCAAATTCTCCCCGGGAAGTAGT
AATGCGATGTGTCATTAGTATCTTCCTTGGTAGGACCAAAAGCTGATTTCATATCAGGGA
AAAAAAGATGTCGAATCTGTGAATTCTCGTCGAGAAACTCTTCGACTAAAGTGGTTGGTG
TTTTTGGTTTCCGTTTGATAGCGCGAGGAGAACGCTTCCTAGGAGGCGGCATATGGATAT
TCTCTTCTTCAACGCGTTCGAGCTCACGGACAATCGGATGATGGATAAGTAACTGAGGTG
AAAGATCAAGATCTACACTATAACTTCTGCTATATAACATGGTTAAATGAATCATGAAAA
CACATCCCACAACTGTAGACAAATTGACGCTTAGACAACTTAATAACACATTGCGGACGT
TTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCC
TTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGG
CGAAAATCCTGTTTGATGGTGGTTCC
   
Sequence Name 49-K118239-SALK_087519C-R210
GenBank Accession LN485489 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K118239-SALK_087519C-R210
GTCATTTGTCTACGTTGTGGGATGTGTTTTCATGATTCATTTAACCATGTTATATAGCAG
AAGTTATAGTGTAGATCTTGATCTTTCACCTCAGTTACTTATCCATCATCCGATTGTCCG
TGAGCTCGAACGCGTTGAAGAAGAGAATATCCATATGCCGCCTCCTAGGAAGCGTTCTCC
TCGCGCTATCAAACGGAAACCAAAAACACCAACCACTTTAGTCGAAGAGTTTCTCGACGA
GAATTCACAGATTCGACATCTTTTTTTCCCTGATATGAAATCAGCTTTTGGTCCTACCAA
GGAAGATACTAATGACACATCGCATTACTACTTCCCGGGGAGAATTTGGACAGACACGGA
AGGGAATCCGATTCAAGCACACGGTGGTGGCATTTTGTTTGATGACATATCAAAGGTTTA
CTATTGGTATGGTGAATATAAAGATGGACCAACTTATCTTTCTCACAAGAAAGGTGCTGC
TAGAGTAAGTTTTAACCGTTTCTTGCTTTCCATTAATGTCTCACTCTTGGACTTTAGAAT
TAGAATGTGAAAACAGCTTCTTGGATTTGTTTCAGGTTGATATAATCGGTGTTGGATGTT
ACTCGTCGAAAGATTTATGGACATGGAAAAAACGAAG

Last Updated on 06.12.2018 16:11