Sequences from confirmation amplicon


Confirmation sequences for line SALK_087753c and hit At1g31940 are shown.
Line ID SALK_087753c
Locus At1g31940 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:11470381
Primers used for PCR 20rb - LBb3, show primer details
   
Sequence Name 65-K116104-SALK_087753C-20rb
GenBank Accession LN485881 [GenBank]
Sequencing primer gene specific primer, 20rb: 5'-TCGGATTTGAAATACCCAGATTAG-3'
Sequence (A. th genome BLAST matches underlined) 
>65-K116104-SALK_087753C-20rb
GCTTGATTCGTTAGCTTGCCCTAAAGCTTTGGCCTTTTTCATTGTACGAATTGGGTTTTG
TTAGATTAATCTTAGTTATTGAAAAATGTCTTACTTTGACCAGGATGATAAGGAAGTATT
GGCTCCAAATTCTGATGTTATTGCAGCAACCACCACAACTGCAACTACTGCTACTCATGG
GATTGAAGTAGCTACAGAGTTTAAACCGGTGGAACATCCGGTCGAGCCGTTAGACAACGA
TCTACCGATTCAGTGTCCATTACCTGAACCATCCATTCTAAATGTAACATCCATGAAAAA
GATGGTTCTTTGTTGTTCTGTTTATAGAAAGCTCTTAGTTTTGAGTTTGTAAACGTTTTT
GGGAATTTTTGATGAAACAGGATGGGAGAATCTGGAAGGAGAGAGTATCAGCGTCTATGA
GGAGAAAAGGTGATTTGCAGATTGTGAAAGATGAGGCTGCTTCTGAGTCTGATGGCTCAG
CACCGAAACCGCCTCGCCCGCCTCAGCCAAATCGTTCGATCTTAATAACACATTGCGGAC
GTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGC
CCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCA
GGCGAAAATCCTGTTTGATGGTGGTTCCGAA
   
Sequence Name 60-K116103-SALK_087753C-R210
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>60-K116103-SALK_087753C-R210
AGCTCTCTTTGAACCCAACAACAACGAACCTTCTTTTGGATGGATGTCTACATTTAGCCG
GGATGGGTCACGAAATGGATACTGAATCGGTTGATCGTTGTCTAACGGCTCAACCGGATG
TTCCGCCGGAGAAAACTCTGTAGCGACCTCTATCCCAAGAGCACCAGTAGTTGCATTTGC
GGTGGTTGCTGCGA

Last Updated on 06.12.2018 16:11