Sequences from confirmation amplicon


Confirmation sequences for line SALK_087857c and hit At4g13840 are shown.
Line ID SALK_087857c
Locus At4g13840 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:8015496
Primers used for PCR 22jr - LBb3, show primer details
   
Sequence Name 60-K113814-SALK_087857C-R210
GenBank Accession LN485743 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>60-K113814-SALK_087857C-R210
TTTGTTACCCACAATATATCTTTGTCTTTCTTGATTAGGGCATATGGTTCGAAAGACAAG
TGAAACCCAATATAGATTTCTAACCTAGTTTAGCTAATAATTTGCCTTTTTTCCGACTCT
ATAAATCAGACCCCATCAACATGGTGTGTCCTTTTTTCTGTCAAATATATATTAAAGATT
GAATAATTCTCTTTTTGGTACAGGGTTCAAGATTGAATAATGCATACCAATACCATATCA
TGTATATATAACCATTTTTAGAACTTTTGTATATTCAAATTTGTCTCAGTACCAATATTA
AATTTGTGTATGATATTTAGACAGTGAGCAATATTATGCATGTGTGAACTATATAAGGAA
TATAGCAAACTAAATTAAGAAGATAAAATTGGTGTAGTCAGATTATACCTGAACGTAGAT
CAAAGGAGAGAACGTCAATTCCGGTCCAATAGGATGATGATAAACCAGAAACTCATCAAC
TGACCGGTCCGGTTTACTGAGCCACTCCTCCACAGTGAGATTGCATTGACCTTCCACGAA
ACGTGTGCCGCAGTCGTTGCACTTGATGTACGGACGGCCCGAGTCTCTCCTTGAGAACCT
GCCTGTGGTCCAAGCGATCTGGTCGAACAACATGAACATGGCCTCCTTCAGGTGTCTCAC
GGTTAAGTCACGTGCTGTCTCGGCTGATAAAATGTATG
   
Sequence Name 73-K113815-SALK_087857C-22jr
GenBank Accession LN486079 [GenBank]
Sequencing primer gene specific primer, 22jr: 5'-CATACATTTACTCAGCCGAGACAG-3'
Sequence (A. th genome BLAST matches underlined) 
>73-K113815-SALK_087857C-22jr
TCATGTTGTTCGACCAGATCGCTTGGACCACAGGCAGGGTTCTCAAGGAGAGACTCGGGC
CGTCCGTACATCAAGTGCAACGACTGCGGCACACGTTTCGTGGAAGGTCAATGCAATCTC
ACTGTGGAGGAGTGGCTCAGTAAACCGGACCGGTCAGTTGATGAGTTTCTGGTTTATCAT
CATCCTATTGGACCGGAATTGACGTTCTCTCCTTTGATCTACGTTCAGGTATAATCTGAC
TACACCAATTTTATCTTCTTAATTTAGTTTGCTATATTCCTTATATAGTTCACACATGCA
TAATATTGCTCACTGTCTAAATATCATACACAAATTTAATATTGGTACTGAGACAAATTT
GAATATACAAAAGTTCTAAAAATGGTTATATATACATGATATGGTATTGGTATGCATTAT
TCAATCTTGAACCCTGTACCAAAAAGAGAATTATTCAATCTTTAATATATATTTGACAGA
AAAAAGGACACACCATGTTGATGGGGTCTGATTTATAGAGTCGGAAAAAAGGCAAATTAT
TAGCTAAACTAGGTTAGAAATCTATATTGGGTTTCACTTGTCTTTCGAACCATATGCCCT
AATCAAGAAAGACAAAGATATATTGTGGTGTAAACAAATTGACGCTNAGACAACTTAATA
ACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGC
AACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTG
GTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTC

Last Updated on 06.12.2018 16:11