Sequences from confirmation amplicon


Confirmation sequences for line SALK_088429c and hit At5g08020 are shown.
Line ID SALK_088429c
Locus At5g08020 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:2573875
Primers used for PCR 50kk - LBb3, show primer details
   
Sequence Name 08-K114130-SALK_088429C-R210
GenBank Accession LN484496 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K114130-SALK_088429C-R210
TTTGTTCCCGGTTTACATAATGCATATATAGCATTAACACTAAAATGAAAAGTATAGATT
TACCTTGAAAAGCTCCAACTTTAAGAGATTTAATCGCGATAACAGGGTGATTATCAGCCA
TGTCCAGCAGTTCTTGGCCAATGCCAGTTGCTAGATCATTCCAAAGGGACACCACAACAG
TTTTCTTTGTCTCATCAGCCAAAGTTATATCTCTCTTTGGGATCATCTCATTGTCGTTCT
TCCTTCTAATGCTCATGGTAGGAGAAACACTTTGAACAACACCGATNACATCTGGAAATG
AGAAACATTAAAGTCACTAACTAAGTTTCATCATAAACCAATGTTTACAAACAAAAAGAG
TTCAATATAGATAACAGCTCTNACCAATAAGGTCTTTCTGATTCACGTATGTACCCAACT
CATCAATGGGTACAAAGTTGAATTTAGTTTCAGGGGTAAACATTTCTTC
   
Sequence Name 18-K114131-SALK_088429C-50kk
GenBank Accession LN484784 [GenBank]
Sequencing primer gene specific primer, 50kk: 5'-TGAAGAAATGTTTACCCCTGAAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K114131-SALK_088429C-50kk
GGGTCATACGTGAATCGAAAGACCTTATTGGTAAGAGCTGTTATCTATATTGAACTCTTT
TTGTTTGTAAACATTGGTTTATGATGAAACTTAGTTAGTGACTTTAATGTTTCTCATTTC
CAGATGTTATCGGTGTTGTTCAAAGTGTTTCTCCTACCATGAGCATTAGAAGGAAGAACG
ACAATGAGATGATCCCAAAGAGAGATATAACTTTGGCTGATGAGACAAAGAAAACTGTTG
TGGTGTCCCTTTGGAATGATCTAGCAACTGGCATTGGCCAAGAACTGCTGGACATGGCTG
ATAATCACCCTGTTATCGCGATTAAATCTCTTAAAGTTGGAGCTTTTCAAGGTAAATCTA
TACTTTTCATTTTAGTGTTAATGCTATATATGCATTATGTTAAACCGGGAACAAATTGAC
GCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTT
TCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCA
GCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCGA

Last Updated on 06.12.2018 16:11