Sequences from confirmation amplicon


Confirmation sequences for line SALK_088542c and hit At2g23380 are shown.
Line ID SALK_088542c
Locus At2g23380 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:9959382
Primers used for PCR 36an - LBb3, show primer details
   
Sequence Name 83-K107675-SALK_088542C-36an
GenBank Accession LN486255 [GenBank]
Sequencing primer gene specific primer, 36an: 5'-CTGCTGCATATCATTCAATTAGGA-3'
Sequence (A. th genome BLAST matches underlined) 
>83-K107675-SALK_088542C-36an
GACAGCCCGCCGTCGTTTATCCATGTAACTGCAAATTGCTTGTGGGAAGGAATGTCCTTG
TTTGCTAAACGGGACTTGCTGCGAGAAGTACTGCGGGTAATGTAATCTATGATTATCCAC
ATCTGTATTTCTTAGCTTTGAATCTCATTACCAACAAACTATATTTTCTGTGTAGTTGCC
CAAAGAGCTGCAAGAATAGGTTTAGAGGTTGTCATTGTGCCAAAAGTCAGTGTCGAAGCC
GCCAGTGTCCATGCTTTGCTGCAGATCGGGAATGTGACCCAGATGTTTGTAGAAACTGCT
GGGTCATGTAAGTCTTATTGGGGATGCAAATCCATCCTAATTGGTTTCTTAGAAAATGCT
CAAGAAATGCAACTTTTTTTTTAGTAGTTCCAGATGCACTTATACTGTGTCTGAATGAAT
TTGAATGTGTAGTGGTGGGGATGGTTCGCTTGGGGTCCCAAGCCAAAGAGGCGATAATTA
TGAGTGCAGGAATATGAAATTGCTCCTGAAACAACAACAAAGGGTAAATACTTAGCCTTG
TTAACTAAATATCTATGATCTGCAATCAAATGATTAAACATGAAATGGCTAAATTTCCTC
CTCTTTTTTATGTTAATTTCAGGATATATTGTGGTGTAAACAAATTGACGCTTAGACAAC
TTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAG
ACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCC
ACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCGAATCGGGCCAA
   
Sequence Name 81-K107674-SALK_088542C-R210
GenBank Accession LN486230 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>81-K107674-SALK_088542C-R210
CATTTGTTACCCACAATATATCCTGAAATTAACATAAAAAAGAGGAGGAAATTTAGCCAT
TTCATGTTTAATCATTTGATTGCAGATCATAGATATTTAGTTAACAAGGCTAAGTATTTA
CCCTTTGTTGTTGTTTCAGGAGCAATTTCATATTCCTGCACTCATAATTATCGCCTCTTT
GGCTTGGGACCCCAAGCGAACCATCCCCACCACTACACATTCAAATTCATTCAGACACAG
TATAAGTGCATCTGGAACTACTAAAAAAAAAGTTGCATTTCTTGAGCATTTTCTAAGAAA
CCAATTAGGATGGATTTGCATCCCCAATAAGACTTACATGACCCAGCAGTTTCTACAAAC
ATCTGGGTCACATTCCCGATCTGCAGCAAAGCATGGACACTGGCGGCTTCGACACTGACT
TTTGGCACAATGACAACCTCTAAACCTATTCTTGCAGCTCTTTGGGCAACTACACAGAAA
ATATAGTTTGTTGGTAATGAGATTCAAAGCTAAGAAATACAGATGTGGATAATCATAGAT
TACATTACCCGCAGTACTTCTCGCAGCAAGTCCCGTTTAGCAAACAAGGACATTCCTTCC
CACAAGCAATTTTGCAGTTACATGGATTAAACTGACGGCAGGGCTGGTCTTTCTTCTCAG
TAATTCTTTTCCTAATTG

Last Updated on 06.12.2018 16:11