Sequences from confirmation amplicon


Confirmation sequences for line SALK_089798c and hit At3g25520 are shown.
Line ID SALK_089798c
Locus At3g25520 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:9269983
Primers used for PCR 12kt - LBb3, show primer details
   
Sequence Name 71-K113949-SALK_089798C-12kt
GenBank Accession LN486034 [GenBank]
Sequencing primer gene specific primer, 12kt: 5'-TGATTCTGTCTTTATGGCTTAC-3'
Sequence (A. th genome BLAST matches underlined) 
>71-K113949-SALK_089798C-12kt
GCCTTCTTTTGGCTGCCGTGTTTTAAGATGTTGGAAACGGATGACGAGTATGAGGGAAAC
GTTGAGGTATGTTGTGTGATTGGTTTCATCTTGATAATAAGAAGCATTGTTATTGTCTCT
TGTTAGAAACTCAAGTCACACAATGAATCAGGCCACTGGAGAAGACTTTTCCGTTGAGCC
AACTGATTCAAGGAGACCTTTCCGTGCTCTTCTTGATGTTGGACTTATCAGGACCACAAC
AGGAAACCGTGTGTTCGGTGCTCTTAAGGTATTAACTTGTTTCTCTTTTTTTGTTGTGAG
TGCTCTTATTTGTCATTCTTCTTGAGCAAATGCTTATACTGGCAATTCTTTAATATCCTT
TTGGTTCGCTGTTTGAATCTGTAGGGTGCTTTGGATGGTGGTCTTGATATCCCTCACAGT
GACAAGAGATTTGCTGGGTTCCACAAGGAGAACAAGCAACTTGATGCTGAAATCCACAGC
ATCTATGAGCCACTGTCTATGAACAAATTGACCACAGGATATATTGTGGTGTAAACAAAT
TGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTT
CTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGT
TGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTT
CC
   
Sequence Name 60-K113948-SALK_089798C-R210
GenBank Accession LN485746 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>60-K113948-SALK_089798C-R210
CACAATATATCCTGTGGTCAATTTGTTCATAGACAGTGGCTCATAGATGCTGTGGATTTC
AGCATCAAGTTGCTTGTTCTCCTTGTGGAACCCAGCAAATCTCTTGTCACTGTGAGGGAT
ATCAAGACCACCATCCAAAGCACCCTACAGATTCAAACAGCGAACCAAAAGGATATTAAA
GAATTGCCAGTATAAGCATTTGCTCAAGAAGAATGACAAATAAGAGCACTCACAACAAAA
AAAGAGAAACAAGTTAATACCTTAAGAGCACCGAACACACGGTTTCCTGTTGTGGTCCTG
ATAAGTCCAACATCAAGAAGAGCACGGAAAGGTCTCCTTGAATCAGTTGGCTCAACGGAA
AAGTCTTCTCCAGTGGCCTGATTCATTGTGTGACTTGAGTTTCTAACAAGAGACAATAAC
AATGCTTCTTATTATCAAGATGAAACCAATCACACAACATACCTCAACGTTTCCCTCATA
CTCGTCATCCATTTCCAACATCTTTAAAACACGGCGAGCCAAAAGAAGGCCAGTACAGTA
AGCTAGTTCAACAAAAAAAAATTACACATAAGTAAGCCATAA

Last Updated on 06.12.2018 16:11