Sequences from confirmation amplicon


Confirmation sequences for line SALK_090068 and hit At5g63630 are shown.
Line ID SALK_090068
Locus At5g63630 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:25474425
Primers used for PCR 32mv - LBb3, show primer details
   
Sequence Name 19-K114624-SALK_090068C-32mv
GenBank Accession LN484822 [GenBank]
Sequencing primer gene specific primer, 32mv: 5'-GATATTGGGGATTTGCTTAGTGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K114624-SALK_090068C-32mv
TGACTTTCTTAGAAGAAGCTGTTTCTGCTTTTGGGTTTGATAAGGAGAATGTTATAGAAG
CGGATAAAACGAGGAATGCAAATGATTCTTATTTGACTAAGACAAGGTAAACAAACATTG
TTGCAAATCTAGTTTCTTCTGTGTGATTTTGAATTAATCACATCAACCTTAAGAGTTTTG
CTTGATTGTGAATCTTGACCTCTTGGTACTGTCAGATTGAGAGTTACTCTTTTCGGTTAA
CAGATTTGATCACTATCCATTGTCTCCCTTATCGCTAAAGGCAATCAAGGATGCTGGATA
CGAGACAATGACTGTTGTTCAGGAAGCTACTCTCCCTATAATTCTCAAAGGTATTTGTTT
TTTTTCTTTTCGTTTCTTATGGTGCTCATTGGATCAACAATGCAGAAACAAATAACACAA
TGATTTAGAAATGGTGACTCTCTAGTCTCTACCAAGCTTTATATAATAACCACCAGTTTA
GCAGAAAGAAAAGCCTAGACACTTTGTGAACTTTCTTGAGTATCAATGGGATTCCTCAAG
TATACAATGACTTCCATGTTATATCTTTTATTCTTTAATCCCTTGGCATCCTCTGTTCTT
TCAGGCAAGGATGTCCTAGCTAAGGCCAAAACAGGAACTGGGAAAACTGTAGCGTTTTTG
GTGAGACATACCATACACTAAGTTGGCCATTTGGTAATGGATTGAACCTATATTTGGTGT
AAACAAATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGG
TGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCT
GAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGA
TGGTGGTT
   
Sequence Name 03-K114623-SALK_090068C-R210
GenBank Accession LN484347 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K114623-SALK_090068C-R210
TTTGTTTACCCAATATAGGTTCATCCATTACCAAATGGCCAACTTAGTGTATGGTATGTC
TCACCAAAAACGCTACAGTTTTCCCAGTTCCTGTTTTGGCCTTAGCTAGGACATCCTTGC
CTGAAAGAACAGAGGATGCCAAGGGATTAAAGAATAAAAGATATAACATGGAAGTCATTG
TATACTTGAGGAATCCCATTGATACTCAAGAAAGTTCACAAAGTGTCTAGGCTTTTCTTT
CTGCTAAACTGGTGGTTATTATATAAAGCTTGGTAGAGACTAGAGAGTCACCATTTCTAA
ATCATTGTGTTATTTGTTTCTGCATTGTTGATCCAATGAGCACCATAAGAAACGAAAAGA
AAAAAAACAAATACCTTTGAGAATTATAGGGAGAGTAGCTTCCTGAACAACAGTCATTGT
CTCGTATCCAGCATCCTTGATTGCCTTTAGCGATAAGGGAGACAATGGATAGTGATCAAA
TCTGTTAACCGAAAAGAGTAACTCTCAATCTGACAGTACCAAGAGGTCAAGATTCACAAT
CAAGCAAAACTCTTAAGGTTGATGTGATTAATTCAAAATCACACAGAAGAAACTAGATTT
GCAACAATGTTTGTTTACCTTGTCTTAGTCAAATAAGAATCATTTGCATTCCTCGTTTTA
TCCGCTTCTATAACATTCTCCTTATCAAACCCAAAAGCAGAAACAGCTTTCTTCTTAAGA
AAGTCATAATCTTGGTCCTCTTCCTCATCATCTTCACTAAGCAATCCCCCC

Last Updated on 06.12.2018 16:11