Sequences from confirmation amplicon


Confirmation sequences for line SALK_090247c and hit At1g55740 are shown.
Line ID SALK_090247c
Locus At1g55740 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:20835442
Primers used for PCR 39db - LBb3, show primer details
   
Sequence Name 18-K109819-SALK_090247C-39db
GenBank Accession LN484774 [GenBank]
Sequencing primer gene specific primer, 39db: 5'-ATAAAGTAGCAGCCTTTGAATGGA-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K109819-SALK_090247C-39db
TACACAAACTCAACATCAGTCTAAATTACTCTGATTATACTAAAACCTTCACTTTTTTTT
TCCTTTTCTATCAGGAGAGTTAGTGTATCTCCCAAAAGATACATCTTTACCAGTGACATT
GATGCCACGTGAATACGAAGTTTTCACGGTGGTTCCGGTGAAGGAATTTTCCGATGGAAG
TAAATTTGCACCGGTGGGGCTTATGGAGATGTTTAATTCTGGAGGAGCCATTGTGAGTTT
AAGATACGATGACGAGGGGACTAAATTTGTCGTTAGGATGAAACTTCGAGGCAGTGGTTT
GGTTGGTGTTTACTCGTCCGTTCGACGACCACGAAGTGTTACGGTGGATTCAGACGACGT
GGAGTACCGGTATGAACCGGAATCCGGTTTAGTTACGTTTACTTTAGGAGTTCCGGAGAA
AGAATTGTATCTGTGGGACGTGGTCATCCAATAATTATTGATCTGTATAATTGTATCAGT
TCTGTATTGATTTGTTGGTTAATCTGTATGGGTTATCGAACACATTGCGGACAACAAATT
GACGTACAAATTGACACATTGACGGGTAGACAACTTAATAACACATTGCGGACGTTTTTA
ATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCAC
CGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAA
ATCCTGT
   
Sequence Name 02-K109818-SALK_090247C-R210
GenBank Accession LN484302 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K109818-SALK_090247C-R210
CATTTGTACGTCAATTTGTTGTCCGCAATGTGTTCGATAACCCATACAGATTAACCAACA
AATCAATACAGAACTGATACAATTATACAGATCAATAATTATTGGATGACCACGTCCCAC
AGATACAATTCTTTCTCCGGAACTCCTAAAGTAAACGTAACTAAACCGGATTCCGGTTCA
TACCGGTACTCCACGTCGTCTGAATCCACCGTAACACTTCGTGGTCGTCGAACGGACGAG
TAAACACCAACCAAACCACTGCCTCGAAGTTTCATCCTAACGACAAATTTAGTCCCCTCG
TCATCGTATCTTAAACTCACAATGGCTCCTCCAGAATTAAACATCTCCATAAGCCCCACC
GGTGCAAATTTACTTCCATCGGAAAATTCCTTCACCGGAACCACCGTGAAAACTTCGTAT
TCACGTGGCATCAATGTCACTGGTAAAGATGTATCTTTTGGGAGATACACTAACTCTCCT
GATAGAAAAGGAAAAAAAAAGTGAAGGTTTTAGTATAATCAGAGTAATTTAGACTGATGT
TTGAGTTTGTGTACTACCTCTGAGATGGGAATAGACAATGGAATCTCCTGTCCATTCAAA
GGCT

Last Updated on 06.12.2018 16:11