Sequences from confirmation amplicon


Confirmation sequences for line SALK_091291c and hit At2g45140 are shown.
Line ID SALK_091291c
Locus At2g45140 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:18612159
Primers used for PCR 63fr - LBb3, show primer details
   
Sequence Name 06-K112347-SALK_091291C-R210
GenBank Accession LN484431 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K112347-SALK_091291C-R210
TTTGGTGAGGATAGTCTTTTTTTTGTTGAAGTTCAGATCATTCCAGTTATTAGCAACTTA
ATGGCTCTTTTATTGTACACTGCTTGATGTGAAATTCTCAAACCTATTAGGCCAATAATC
TTAACTTTTTGTTTATTACTGAAGAATTCTGAATAATCTTAACCTTGTTTGAATGAAGCT
TTAACTAATTTCTGAAAATACTGTCGTCCAGAAGAATATTGTTTCCTCTGCTGTAGTTTT
AGCTTTTTACTGATTATGCATGCAATTGAATTGGTCTAAGCTGCAACATATCTACTAGCT
ATTAATTGGCACATTTTAGCATTGAAGTTACGAGACCTGAGTGATCATTCTATTACAATG
TAATTTTGACACCTATCAATTTGTATGATATCAGGCAAGAGCTCTGGTCACAAAACTCAC
CGAGGAAAAGAACTCTGCGGTTCAACTGAACAACAGGCTTCAACAAGAATTGGTAAGCAA
CTTTTAAACTATTCCTTCCGGTGGTTGGTTTAAGAATCAAAGA
   
Sequence Name 15-K112348-SALK_091291C-63fr
GenBank Accession LN484696 [GenBank]
Sequencing primer gene specific primer, 63fr: 5'-TCTTTGATTCTTAAACCAACCACC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K112348-SALK_091291C-63fr
AGTGCTACCATTCTTGTTGAGCCTGTTGTTCGTTGAACCGCAGAGTTCTTTTCCTCGGTG
CGTTTTGTGACCAGAGCTCTTGCCTGATATCATACAAATTGATAGGTGTCAAAATTACAT
TGTAATAGAATGATCACTCAGGTCTCGTAACTTCAATGCTAAAATGTGCCAATTAATAGC
TAGTAGATATGTTGCAGCTTAGACCAATTCAATTGCATGCATAATCAGTAAAAAGCTAAA
ACTACAGCAGAGGAAACAATATTCTTCTGGACGACAGTATTTTCAGAAATTAGTTAAAGC
TTCATTCAAACAAGGTTAAGATTATTCAGAATTCTTCAGTAATAAACAAAAAGTTAAGAT
TATTGGCCTAATAGGTTTGAGAATTTCACATCAAGCAGTGTACAATAAAAGAGCCATTAA
GTTGCTAATAACTGGAATGATCTGAACTTCAACAAAAAAAAGACTAATCCTCACCAAATT
GACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTC
TTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTT
GCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTC
CG

Last Updated on 06.12.2018 16:11