Sequences from confirmation amplicon


Confirmation sequences for line SALK_092571c and hit At5g25350 are shown.
Line ID SALK_092571c
Locus At5g25350 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:8795096
Primers used for PCR 38dr - LBb3, show primer details
   
Sequence Name 72-K110311-SALK_092571C-38dr
GenBank Accession LN486052 [GenBank]
Sequencing primer gene specific primer, 38dr: 5'-ATCTGAAGCATGTCTCTCTGAACA-3'
Sequence (A. th genome BLAST matches underlined) 
>72-K110311-SALK_092571C-38dr
TGTCGCTTTGGCCAATCTGCATTGTCACTTGAAAGTTTGAAACTTGAAGAATGCCACAGG
ATCAACCAGTTTGGTCTTATGGGTTTTCTCATGAACTGTGGCTCAAAGTTGAAAGCTTTC
TCTTTGGCAAACTGTCTGGGCATCAGTGACTTCAACTCAGAATCCTCTCTGCCATCACCC
AGTTGCAGCTCTTTACGTTCTCTGTCAATCCGATGCTGCCCTGGGTTTGGGGATGCAAGT
CTCGCTTTCTTGGGAAAGTTCTGTCATCAGCTTCAGGATGTTGAACTCTGTGGACTAAAC
GGAGTGACAGATGCAGGTGTGCGCGAGTTGCTACAGAGCAACAATGTTGGTCTAGTGAAG
GTGAACCTGAGCGAATGTATCAATGTTTCAGACAACACAGTCTCTGCAATTTCTGTTTGC
CACGGACGCACATTGGAGTCTCTTAACCTTGACGGCTGCAAGAACATCACAAACGCAAGC
CTTGTCGCAGTAGCCAAGAACTGCTACTCAGATTGTGGTGTAAACAAATTGACGCTTAGA
CAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAG
TGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCG
GTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCG
   
Sequence Name 62-K110310-SALK_092571C-R210
GenBank Accession LN485789 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>62-K110310-SALK_092571C-R210
GTCATTTGTTTACCCACAATCTGAGTAGCAGTTCTTGGCTACTGCGACAAGGCTTGCGTT
TGTGATGTTCTTGCAGCCGTCAAGGTTAAGAGACTCCAATGTGCGTCCGTGGCAAACAGA
AATTGCAGAGACTGTGTTGTCTGAAACATTGATACATTCGCTCAGGTTCACCTTCACTAG
ACCAACATTGTTGCTCTGTAGCAACTCGCGCACACCTGCATCTGTCACTCCGTTTAGTCC
ACAGAGTTCAACATCCTGAAGCTGATGACAGAACTTTCCCAAGAAAGCGAGACTTGCATC
CCCAAACCCAGGGCAGCATCGGATTGACAGAGAACGTAAAGAGCTGCAACTGGGTGATGG
CAGAGAGGATTCTGAGTTGAAGTCACTGATGCCCAGACAGTTTGCCAAAGAGAAAGCTTT
CAACTTTGAGCCACAGTTCATGAGAAAACCCATAAGACCAAACTGGTTGATCCTGTGGCA
TTCTTCAAGTTTCAAACTTTCAAGTGACAATGCAGATTTGGCCAAAGCGACAAGTCCTTT
ACCAGAAACAAGCAAGCATTTGTTCAGAGAGACATGCTTC

Last Updated on 06.12.2018 16:11