Sequences from confirmation amplicon


Confirmation sequences for line SALK_092885 and hit At1g20350 are shown.
Line ID SALK_092885
Locus At1g20350 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:7044096
Primers used for PCR 12ei - LBb3, show primer details
   
Sequence Name 52-K111283-SALK_092885C-12ei
GenBank Accession LN485558 [GenBank]
Sequencing primer gene specific primer, 12ei: 5'-ATGGCTCTAAAAGCAAACTCCATA-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K111283-SALK_092885C-12ei
CCGTTGTGATTGGTTGATTTAGATACTGTGTCGTGACATGGCATTATGAGGTGGAGGATG
TTTGTCCACGTTTTAAATAAAGATAAGGTTCTTGAATGACGTGGCGTTTTTATATTAGTA
TTTTGTGATAAAGCGTGTTTACAAATGGGCCTTAATGGGCTTTGACCATGGTCAAGTAAC
GTGAACAACACGATATTTTTCCATTTTTGTAATTTTTCCTCATTCTTAGTTTCGTTTTCA
AGGGCTCTTTTCTTATACCCTAAAAAGTTACTTGTAGATCAAAATCGAAAATCAATGGGA
ACTCCAGAATCATCGAGAGAGCCATGTCCGGATCGGATCCTAGATGATGTCGGAGGTGCG
TTTGCGATGGGTGCTGTTGGTGGATCAGCGTATCACCTCATAAGAGGAATCTACAACTCT
CCCGGCGGAGCTCGTCTCTCCGGCGGCGTTCAAGCTTTGAGAATGAGCGGGCCGAGAAGC
GGAGGAAGCTTCTCCGTGTGGGGTGGTCTTTACTCAACCTTCGATTGTGCGTTGGTGTAC
GCAAGACAAAAGGAAGATCCATGGAACTCGATCTTATCTGGTGCAGCCACTGGAGGATTC
CTCTCGTTGCGTCAAGGCTTAGGCGCGTCTGCTAGATCGGCTTTAGTCGGAGGTAAACAA
ATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTT
TTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGA
GTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGG
TTCCG
   
Sequence Name 36-K111282-SALK_092885C-R210
GenBank Accession LN485234 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K111282-SALK_092885C-R210
GTCATTTGTTACCTCCGACTAAGCCGATCTAGCAGACGCGCCTAAGCCTTGACGCAACGA
GAGGAATCCTCCAGTGGCTGCACCAGATAAGATCGAGTTCCATGGATCTTCCTTTTGTCT
TGCGTACACCAACGCACAATCGAAGGTTGAGTAAAGACCACCCCACACGGAGAAGCTTCC
TCCGCTTCTCGGCCCGCTCATTCTCAAAGCTTGAACGCCGCCGGAGAGACGAGCTCCGCC
GGGAGAGTTGTAGATTCCTCTTATGAGGTGATACGCTGATCCACCAACAGCACCCATCGC
AAACGCACCTCCGACATCATCTAGGATCCGATCCGGACATGGCTCTCTCGATGATTCTGG
AGTTCCCATTGATTTTCGATTTTGATCTACAAGTAACTTTTTAGGGTATAAGAAAAGAGC
CCTTGAAAACGAAACTAAGAATGAGGAAAAATTACAAAAATGGAAAAATATCGTGTTGTT
CACGTTACTTGACCATGGTCAAAGCCCATTAAGGCCCATTTGTAAACACGCTTTATCACA
AAATACTAATATAAAAACGCCACGTCATTCAAGAACCTTATCTTTATTTAAAACGTGGAC
AAACATCCTCCACCTCATAATGCCATGTCACGACACAGTATCTAAAATCAACCAATCACA
ACGCGTCTTTATAGATAACTTGTTTTTTTATGGAGTTTGC

Last Updated on 06.12.2018 16:11