Sequences from confirmation amplicon


Confirmation sequences for line SALK_093085c and hit At1g50400 are shown.
Line ID SALK_093085c
Locus At1g50400 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:18668968
Primers used for PCR 52fm - LBb3, show primer details
   
Sequence Name 84-K111509-SALK_093085C-52fm
GenBank Accession LN486271 [GenBank]
Sequencing primer gene specific primer, 52fm: 5'-GAGGCAACCTGTTGGATAATAAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>84-K111509-SALK_093085C-52fm
ATCATACTCAAGTTAGCTTGAAGATTGTTGGAATATTGTAAACCAACATAGAAAAGAAGA
ATCGATTTTCTGATTGAGTTGCCTTATTCTAACTAAGAAGAAACATTTACCGTTTTGTCA
GTCTCATATCGAGCTGCATAACCAACACCCGACATCAGTTGCTGACCAAGCCAGAAAGCC
TCACCACCCAAAGACAGGTGTGGTGTCACATGCTGGAAGAAAAAGATATGATTCAGTGAA
AAGAAACGAATATCTATTTAGCAACTAACAAAAGATTAACTAATGCTGACAAAATAAACA
AAAGCTTGTCCAGGGATAAACGGAAAAGCTACCTAACTATAGACTTAACCACAGAATTTT
AGTAGAACAGTTTAAAATGGTAAAGCATAGTCACTTTACCTGAATATAGTTAGCTGCATA
AACGGAATTGTTTCCAAGCTGAAGCTGAGTTCTGTAGTCAGACCCCTAAATAGGTGTAAA
CCGTGAATTGTCAAAACCAAATAAAAGCTAAAAGGAAAAACATACTGAACAGAGAAAAGC
CCTCACCTTGTAATCAATGACGAGGTGGCCTTGCGACTTATCTTCTTCATCTGTCAGCTG
GCAGAGAAAAGAAACAATATGGTTATAGATAGAAAGAAAACATTATGCAGCTACTACAAC
AAAGACCAGACACACAATAATTTCTGGTTACCAGAGCATTAGCCTTGACGGAAAAATTGT
CAGTTAAATCGAACTTGACGCTNAGACAACTTAATAACACATTGTGGTGTATATTGTGGT
GTAAACAAATTGACGCTNAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGG
GGTGGTT
   
Sequence Name 70-K111508-SALK_093085C-R210
GenBank Accession LN486003 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>70-K111508-SALK_093085C-R210
CATTTGTTACACCACAATATACACCACAATGTGTTATTAAGTTGTCTAAGCGTCAAGTTC
GATTTAACTGACAATTTTTCCGTCAAGGCTAATGCTCTGGTAACCAGAAATTATTGTGTG
TCTGGTCTTTGTTGTAGTAGCTGCATAATGTTTTCTTTCTATCTATAACCATATTGTTTC
TTTTCTCTGCCAGCTGACAGATGAAGAAGATAAGTCGCAAGGCCACCTCGTCATTGATTA
CAAGGTGAGGGCTTTTCTCTGTTCAGTATGTTTTTCCTTTTAGCTTTTATTTGGTTTTGA
CAATTCACGGTTTACACCTATTTAGGGGTCTGACTACAGAACTCAGCTTCAGCTTGGAAA
CAATTCCGTTTATGCAGCTAACTATATTCAGGTAAAGTGACTATGCTTTACCATTTTAAA
CTGTTCTACTAAAATTCTGTGGTTAAGTCTATAGTTAGGTAGCTTTTCCGTTTATCCCTG
GACAAGCTTTTGTTTATTTTGTCAGCATTAGTTAATCTTTTGTTAGTTGCTAAATAGATA
TTCGTTTCTTTTCACTGAATCATATCTTTTTCTTCCAGCATGTGACACCACACCTGTCTT
TGGGTGGTGAGGCTTTCTGGCTTGGTCAGCAACTGATGTCGGGTGTTGGTTATGCAGCTC
GATATGAGACTGACAAAACGGTAAATGTTTCTTCTTAGTTAGAATAAGGCAACTCAATCA
GAAAATCGATTCTTCTTTTCTATGTTGGTTTACAATATTCCAACAATCTTCAAGCTAACT
TGAGTATGATCCTTTTCATTTTTTTGAATTTCTTTAAATGTTTTATTATCC

Last Updated on 06.12.2018 16:11