Sequences from confirmation amplicon


Confirmation sequences for line SALK_093414 and hit At1g53280 are shown.
Line ID SALK_093414
Locus At1g53280 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:19866221
Primers used for PCR 34ea - LBb3, show primer details
   
Sequence Name 68-K110715-SALK_093414C-34ea
GenBank Accession LN485950 [GenBank]
Sequencing primer gene specific primer, 34ea: 5'-TTGAGTCAAGAGTGGAAATTGATG-3'
Sequence (A. th genome BLAST matches underlined) 
>68-K110715-SALK_093414C-34ea
GAATTCTCTGTCACGCTTGTGGAGCAACTTCTTGGGAAAGAGAAAGCTGTTGAAGTCTCT
GGCCCCTTGGTATGTCTTATCCCTGACTCTTCTATCAACCTTAATTTGGTCTCCAAATAA
TCGACAGATCTTAGAGGCCAGTTTCTGTTGAGAAGATGGCTCTCCGCAAAGAAGGATATA
TTTAGTAGCTGATTCGAGGGGAGCACTTATTTGATTTAACACTCTTTAAACAAATTGACG
CTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTT
CACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAG
CAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCGAA
ATCGGCAAAA
   
Sequence Name 52-K110714-SALK_093414C-R210
GenBank Accession LN485557 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K110714-SALK_093414C-R210
CGTCATTTGTTTAAGAGTGTTAAATCAATAAGTGCTCCCCTCGAATCAGCTACTAAATAT
ATCCTTCTTTGCGGAGAGCCATCTTCTCAACAGAAACTGGCCTCTAAGATCTGTCGATTA
TTTGGAGACCAAATTAAGGTTGATAGAAGAGTCAGGGATAAGACATACCAAGGGGCCAGA
GACTTCAACAGCTTTCTCTTTCCCAAGAAGTTGCTCCACAAGCGTGACAGAGAATTCCAT
GGTAGTTCCAGGCCCTCTACTGGTCACTATCTTCCCATCAATTTCCACTCTTGACTCA

Last Updated on 06.12.2018 16:11