Sequences from confirmation amplicon


Confirmation sequences for line SALK_093974 and hit At4g05280 are shown.
Line ID SALK_093974
Locus At4g05280 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:2698491
Primers used for PCR BN65 - R204, show primer details
   
Sequence Name 70-K107371-SALK_093974C-BN65
GenBank Accession LN485997 [GenBank]
Sequencing primer gene specific primer, BN65: 5'-GAAGGAGAAGCGGGTATTAAGAGT-3'
Sequence (A. th genome BLAST matches underlined) 
>70-K107371-SALK_093974C-BN65
GCACGGAGGAACTGGGTTAGAATCTCTAATTTACCGTTTGTTGTCTTTGGGTGTATCAGA
ATTGTAAAGCTTGTTTGATTAAGGGGTTAGAATTTGTCGGAATTTAGCATATCTTTAGGA
TGAAAAATTGAGAAGTAGAATTGGAAATCTCTGTTTCCGCCAAAAATATGATTTTGAGTT
ATAATCTGGAAAATGTCTGTGTACGTACACTACTTCGGTTTGCTTAACAGTATATGACTT
TTAATTGCAGGTAGTCCAAGTGTTGCTCCGACTGAAGATCTTACAGTTGAAGAGTTACCA
CCAAGACTCTTTGCTTTAGACCGGTACCCTTCCGAGACGAAGATGAACGCGTACTCTAAA
CCGGAGTACATTTCTGACATAGCAAATGTACTAAAGGGGAAACCAGAGATGCAGTTTCTT
CTCGATTCTCCATTTGGGGAACTTTTTAAAATACCCAAAAACAAGGCATCTTTCAATGCT
AAGCTTGTCCTTAGACTAATATGCCGTAAGTTAGTGACAAAGAAGGTGAATGAGATGTGG
ATTGTATTCGGCGGTCATCCAATCAGATTTGGATTAAGAGAATTTAGTATCTTAACTGGG
CTGGAATGTGGGAAGTATCCCAAGAAGAAAGACGTTGATGATGTTATCAGTGTGAAACCT
GAGTGTGAGAGTGTTTGGAAGACTCTGTTTGATGAGAGGTTTTGGGACACGGTTCCAACA
ATTGCTGATTTGGTGAGCTGGCTGTAGGAGGAGGAAAGCATGGAGGGGTGGAAGCAACTG
GCACTATCTCTTTTTATTTTGGTTGACGGTGTGGTGTAAACAAATTGACGCTTAGACAAC
TTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAG
ACGGGCAACAGCTGATTGCCCTTCACCGCCCGGCCC
   
Sequence Name 86-K107372-SALK_093974C-R210
GenBank Accession LN486293 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>86-K107372-SALK_093974C-R210
GTCATTTGTTACCCACACCGTCAACCAAAATAAAAAGAGATAGTGCCAGTTGCTTCCACC
CCTCCATGCTTTCCTCCTCCTACAGCCAGCTCACCAAATCAGCAATTGTTGGAACCGTGT
CCCAAAACCTCTCATCAAACAGAGTCTTCCAAACACTCTCACACTCAGGTTTCACACTGA
TAACATCATCAACGTCTTTCTTCTTGGGATACTTCCCACATTCCAGCCCAGTTAAGATAC
TAAATTCTCTTAATCCAAATCTGATTGGATGACCGCCGAATACAATCCACATCTCATTCA
CCTTCTTTGTCACTAACTTACGGCATATTAGTCTAAGGACAAGCTTAGCATTGAAAGATG
CCTTGTTTTTGGGTATTTTAAAAAGTTCCCCAAATGGAGAATCGAGAAGAAACTGCATCT
CTGGTTTCCCCTTTAGTACATTTGCTATGTCAGAAATGTACTCCGGTTTAGAGTACGCGT
TCATCTTCGTCTCGGAAGGGTACCGGTCTAAAGCAAAGAGTCTTGGTGGTAACTCTTCAA
CTGTAAGATCTTCAGTCGGAGCAACACTTGGACTACCTGCAATTAAAAGTCATATACTGT
TAAGCAAACCGAAGTAGTGTACGTACACAGACATTTTCCAGATTATAACTCAAAATCATA
TTTTTGGCGGAAACAGAGATTTCCAATTCTACTTCTCAATTTTTCATCCTAAAGATATGC
TAAATTCCGACAAATTCTAACCCCTTAATCAAACAAGCTTTACAATTCTGATACACCCAA
AGACAACAAACGGTAAATTAGAGATTCTAACCCAGTTCCTCCGTTGCCTTTGTCGCAGAG
CGTTTTCTCCGCGGGGAAACTCTTATAC
Primers used for PCR 64wa - LBb3, show primer details
   
Sequence Name 83-K118526-SALK_093974C-64wa
GenBank Accession LN486262 [GenBank]
Sequencing primer gene specific primer, 64wa: 5'-TGTAAAGCTTGTTTGATTAAGGGG-3'
Sequence (A. th genome BLAST matches underlined) 
>83-K118526-SALK_093974C-64wa
CTTTAGATGAAATTGAGAATAGAATTGGAAATCTCTGTTTCCGCCAAAATATGATTTTGA
GTTATAATCTGGAAAATGTCTGTGTACGTACACTACTTCGGTTTGCTTAACAGTATATGA
CTTTTAATTGCAGGTAGTCCAAGTGTTGCTCCGACTGAAGATCTTACAGTTGAAGAGTTA
CCACCAAGACTCTTTGCTTTAGACCGGTACCCTTCCGAGACGAAGATGAACGCGTACTCT
AAACCGGAGTACATTTCTGACATAGCAAATGTACTAAAGGGGAAACCAGAGATGCAGTTT
CTTCTCGATTCTCCATTTGGGGAACTTTTTAAAATACCCAAAAACAAGGCATCTTTCAAT
GCTAAGCTTGTCCTTAGACTAATATGCCGTAAGTTAGTGACAAAGAAGGTGAATGAGATG
TGGATTGTATTCGGCGGTCATCCAATCAGATTTGGATTAAGAGAATTTAGTATCTTAACT
GGGCTGGAATGTGGGAAGTATCCCAAGAAGAAAGACGTTGATGATGTTATCAGTGTGAAA
CCTGAGTGTGAGAGTGTTTGGAAGACTCTGTTTGATGAGAGGTTTTGGGACACGGTTCCA
ACAATTGCTGATTTGGTGAGCTGGCTGTAGGAGGAGGAAAGCATGGAGGGGTGGAAGCAA
CTGGCACTATCTCTTTTTATTTTGGTTGACGGTGTGGTGTAAACAAATTGACGCTTAGAC
AACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGT
GAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGG
TCCACGCTGGTTTGCCCCAGCAGGCGAAAATCTGTTTGATGGTGGTTCC
   
Sequence Name 73-K118525-SALK_093974C-R210
GenBank Accession LN486093 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>73-K118525-SALK_093974C-R210
CCAAAATAAAAAGAGATAGTGCCAGTTGCTTCCACCCCTCCATGCTTTCCTCCTCCTACA
GCCAGCTCACCAAATCAGCAATTGTTGGAACCGTGTCCCAAAACCTCTCATCAAACAGAG
TCTTCCAAACACTCTCACACTCAGGTTTCACACTGATAACATCATCAACGTCTTTCTTCT
TGGGATACTTCCCACATTCCAGCCCAGTTAAGATACTAAATTCTCTTAATCCAAATCTGA
TTGGATGACCGCCGAATACAATCCACATCTCATTCACCTTCTTTGTCACTAACTTACGGC
ATATTAGTCTAAGGACAAGCTTAGCATTGAAAGATGCCTTGTTTTTGGGTATTTTAAAAA
GTTCCCCAAATGGAGAATCGAGAAGAAACTGCATCTCTGGTTTCCCCTTTAGTACATTTG
CTATGTCAGAAATGTACTCCGGTTTAGAGTACGCGTTCATCTTCGTCTCGGAAGGGTACC
GGTCTAAAGCAAAGAGTCTTGGTGGTAACTCTTCAACTGTAAGATCTTCAGTCGGAGCAA
CACTTGGACTACCTGCAATTAAAAGTCATATACTGTTAAGCAAACCGAAGTAGTGTACGT
ACACAGACATTTTCCAGATTATAACTCAAAATCATATTTTTGGCGGAAACAGAGATTTCC
AATTCTACTTCTCAATTTTTCATCCTAAAGATATGCTAAATTCCGACAAATTCTAACCCC
TTAATCA

Last Updated on 06.12.2018 16:11