Sequences from confirmation amplicon


Confirmation sequences for line SALK_094648c and hit At1g01380 are shown.
Line ID SALK_094648c
Locus At1g01380 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:147412
Primers used for PCR 01ii - LBb3, show primer details
   
Sequence Name 38-K112623-SALK_094648C-01ii
GenBank Accession LN485263 [GenBank]
Sequencing primer gene specific primer, 01ii: 5'-GAAGCTTTGAATAAAATTTGCGAT-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K112623-SALK_094648C-01ii
ACGTTAATTATTTTATTTCATCCCTAACAAACTTTCAACTCAACTTTTGATAGTTCTAAC
AATATATTTCATTAAATTGACAAAATTTAATTCGATTTGTAATAGATTGGACTTCATAAA
TGATAATCAAGTGATTCTCCTAATGCTTTTTTTCTTCTTGGGAAAAATGAATACATATAT
TGACTCTTTTTTTTCATACATATTTGTACTCAACATAGTTGATTTATATCGACTTAATTA
TGATTTATTCATCTGCGTGTAAATATAAAACATCAAGTAGTATCATTTTAAAAAAAGCAA
TTAGACAAGAACATGACGGAGCAAATGATGTAATGCAACATGTCTTATGATTCTCATTGG
TCCTTAAACGAAATCACTCGAGCATTGCACACATACTGACATATATTGGCTGCCACCCAT
AAGTTGCTAAATTAATTAATAAGAATAATATTAGTTAAATCCGATTTAAGGAAAACAAAG
TACGATATACATCATACACGATTCCTTAATGTGGAAACCGACCAAACCGGTCGTTAAACT
TTGCCCTTATAAATACAGAATACACTTCATGTTCTTCCCTTAAAAATCCATCATTCTCTC
TTCCTCTCTGCTCTCTCTTTACCTTAAACCGTACCAAACCGTATTGATTGATAATATTGC
ACGGTGCGATCGTAAATCTTTGTGTACTAAGGTGTCTTTTGAGATGAATACGCAGCGTAA
GTCGAAGCATCTTAAGACCAATCCAACCATTGTTGCCTCTTCTTCTGAAGGTAACAACTT
TCTCCGCTATCTCATGATCTCCATCCACTATACGGTGTCGTTTGATTTTACTCTTTTTTT
TTTTGAAAAAAAAAAAAGGGAAAATTTG
   
Sequence Name 14-K112622-SALK_094648C-R210
GenBank Accession LN484669 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K112622-SALK_094648C-R210
ATCAAATCCTCTTCTTCCTGAGCCATTGCTATTTCTTCCCACTCAAGACTGCTCACTTCT
GTCCCACGTCATCATCCAAATTTCACATTTATTATTATTTCAAAAAAAAAAAAGGTAAAA
CCCACCCCCCCCCTAAGGGGGAGGGGAATCCGGAAAAACCGGAAAAATTTTTTCCCTTCC
AAAAAAAAGGCCACAAGGGGGGGATGGGCCTTAAAAAGCTTCCACTTACCCGGGGTTTTC
CTCCCAAAAAACCCCTTAATCCCCAAAAATTTCCAACCCCCCCGGGCAAATTTTCCAACC
AAACCGGTTGGGGCCGGTTTAAGGGAAAAAAAAGCCCAAAGGGAAAAAAAAATGGGGGAT
TTTTAGGGGAAAAACCGGAAGGGGTTTCGGGTTTTAAAAGGGCAAATTTTAACCACCGGT
TGGGGCGGTTTCCCCTTTAAGGAACCGGGGAAGAGGGAAACCGAACTTTGTTTCCCCTAA
ACCGGATTTAACCAAAATTTTCCCTATTAATTAATTTACCAACTTTGGGGGGGGCCCCAA
ATATTTTCAATTTGGGGGCAAGGCCCAGGGGATTTCCTTTAGGGGCCAAGGGAAACCCAA
AAAACGGGGGCATTACCCCCTTTGCCCCCCCAGGGCCTGGGCCAATGGCTTTTTTAAAAA
GGAACCCCCCGGGGGTTTTAATTTTCCCCCCCAAAGAAAAAACCCAAATTAAGCCGAATA
AAACCCCCTTTGTTGGGGCCAAATTGGTTGGAAAAAAAAAGACCAAATATGGTTTTCCTT
TTTCCCAGAAAAAAAAAAACCCT

Last Updated on 06.12.2018 16:11