Sequences from confirmation amplicon


Confirmation sequences for line SALK_094930c and hit F4P9 are shown.
Line ID SALK_094930c
Locus F4P9 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:14163163
Primers used for PCR 03en - LBb3, show primer details
   
Sequence Name 16-K111285-SALK_094930C-03en
GenBank Accession LN484719 [GenBank]
Sequencing primer gene specific primer, 03en: 5'-TTGATTCGTTGTGTAAATGGGTTA-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K111285-SALK_094930C-03en
GTATCTAAAATTGTTGCATTGAGGTTGAAGAGAATTGTTTGTTGTTTTACGAGGAAAATA
TTCGTTAATTCTTTAATTAGATGTGTTCAGAAATAGAAAGTCTTTATTTGTTTAAGAAAT
TGTAACAAGTTCTTGCGATGATGAAAAGAATGAGATTTAATGGGCTTTGAGCCCAACTCA
TGTAAAGCCTTTATAGTATATAAGAAGATAAAAATTCGAAGATAAAAACCAAAACCCTAT
CCACTTTACTCTAAACCTCCATTACCTTCGTCTTTCTTCCTCAGTCGCCCTACAATGGCT
TTGCCTTTGTCTGGCACGCGCCACCTCACGCGCGCTCTCCTCTCTAATGTCACTCTTATG
GCTCCTCCTCGGATTCCTTCCTCTGTTCACTACGGTGGATCTCGGTTAGGTTGTTCGACC
CGGTTCTTCTCAATTCGATGTGGAGCTAACCGGTCAGGTTCTACTTACTCGCCGCTTAAC
TCTGGCTCTAACTTTAGTGATCGGCCTCCGACGGAGATGGCTCCTTTGTTTCCGGGATGC
GATTATGAGCACTGGCTTATTGTTATGGATAAACCTGGCGGGGAAGGCGCGACTAAGCAG
CAGATGATTGATTGTTACATTCAAACCCTAGCGAAAGTCGTTGGAAGGTATAATCTTCAG
ATTATATATGTTAATTGTCTAG
   
Sequence Name 05-K111284-SALK_094930C-R210
GenBank Accession LN484397 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K111284-SALK_094930C-R210
CATTTGTTTACCACAATATACCCTAGATTTTTTCATACATTTTTCATTATATTTCAACAG
CAACCTAGACAATTAACATATATAATCTGAAGATTATACCTTCCAACGACTTTCGCTAGG
GTTTGAATGTAACAATCAATCATCTGCTGCTTAGTCGCGCCTTCCCCGCCAGGTTTATCC
ATAACAATAAGCCAGTGCTCATAATCGCATCCCGGAAACAAAGGAGCCATCTCCGTCGGA
GGCCGATCACTAAAGTTAGAGCCAGAGTTAAGCGGCGAGTAAGTAGAACCTGACCGGTTA
GCTCCACATCGAATTGAGAAGAACCGGGTCGAACAACCTAACCGAGATCCACCGTAGTGA
ACAGAGGAAGGAATCCGAGGAGGAGCCATAAGAGTGACATTAGAGAGGAGAGCGCGCGTG
AGGTGGCGCGTGCCAGACAAAGGCAAAGCCATTGTAGGGCGACTGAGGAAGAAAGACGAA
GGTAATGGAGGTTTAGAGTAAAGTGGATAGGGTTTTGGTTTTTATCTTCGAATTTTTATC
TTCTTATATACTATAAAGGCTTTACATGAGTTGGGCTCAAAGCCCATTAAATCTCATTCT
TTTCATCATCGCAAGAACTTGTTACAATTTCTTAAACAAATAAAGACTTTCTATTTCTGA
ACACATCTAATTAAAGAATTAACGAATATTTTCCTCGTAAAACAACAAACAATTCTCTTC
AACCTCAATGCAACAATTTTTAGATTACAAATCAGCCCCAAAAACTCGAAAACCATACTT
TTAACCCATTTACACAACGAATC

Last Updated on 06.12.2018 16:11