Sequences from confirmation amplicon


Confirmation sequences for line SALK_095004c and hit At1g51690 are shown.
Line ID SALK_095004c
Locus At1g51690 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:19167610
Primers used for PCR 26ht - LBb3, show primer details
   
Sequence Name 36-K115696-SALK_095004C-26ht
GenBank Accession LN485237 [GenBank]
Sequencing primer gene specific primer, 26ht: 5'-CAATGGGTGAATAACCTACCTGTT-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K115696-SALK_095004C-26ht
GCATATACTCTTCGACATCTAGCCACAGGGCTCGACTCATGGCTAGTTACCTGTATATGT
CAAATGAACTATTAGTCTACAAATGAGCAATATTAAGTAATATTGCATGACTTAAGAATC
AATTCCTAATCAGTGGTAGTGAAGGCCATGGAATGGGAAAATGCACAAGTACGAAAAACA
CAAGTCCAGCACCAATGAACAGTAAATTTGAGCAAAGTGTATCATTTTTCTGGACGCATA
TACTACATGATATGATCTATATTACTTCGACTCAATTGGCGCCAATGCAAAACCCTGCAT
TTGAGTTTTAGCAATCACCCAGAAAGAGGTTAGATAAATGGTTGGCTCTATGATGCAGGT
TTTACTTAAGTTAGTGCTGAAACAAGCATAAATATTTAGGACAACTTTGACGCTNAGACA
ACTTAATAAAGAAAACATATTGTTCAGGGTAGCAGGGTACCGGATATATTGTGGTGTAAA
CAAATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGG
TTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAG
AGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGG
TGGTTCCGA
   
Sequence Name 25-K115695-SALK_095004C-R210
GenBank Accession LN485008 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K115695-SALK_095004C-R210
TTTGTTACACCACAATATATCCGGTACCCTGCTACCCTGAACAATATGTTTTCTTTATTA
AGTTGTCTAAGCGTCAAAGTTGTCCTAAATATTTATGCTTGTTTCAGCACTAACTTAAGT
AAAACCTGCATCATAGAGCCAACCATTTATCTAACCTCTTTCTGGGTGATTGCTAAAACT
CAAATGCAGGGTTTTGCATTGGCGCCAATTGAGTCGAAGTAATATAGATCATATCATGTA
GTATATGCGTCCAGAAAAATGATACACTTTGCTCAAATTTACTGTTCATTGGTGCTGGAC
TTGTGTTTTTCGTACTTGTGCATTTTCCCATTCCATGGCCTTCACTACCACTGATTAGGA
ATTGATTCTTAAGTCATGCAATATTACTTAATATTGCTCATTTGTAGACTAATAGTTCAT
TTGACATATACAGGTAACTAGCCATGAGTCGAGCCCTGTGGCTAGATGTCGAAGAGTATA
TGCTCATGCTCATGATTATCATATTAATTCAATCTCAAATAACAGGTAGGTTATCACCCC
ATT

Last Updated on 06.12.2018 16:11