Sequences from confirmation amplicon


Confirmation sequences for line SALK_095404c and hit At3g25990 are shown.
Line ID SALK_095404c
Locus At3g25990 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:9506622
Primers used for PCR 61qz - LBb3, show primer details
   
Sequence Name 77-K116055-SALK_095404C-61qz
GenBank Accession LN486178 [GenBank]
Sequencing primer gene specific primer, 61qz: 5'-CCATCTGAGCCTTTCTCTTCTCTA-3'
Sequence (A. th genome BLAST matches underlined) 
>77-K116055-SALK_095404C-61qz
GAGATGATCTTCACTATCTCTAGAACCTTTTTTTTTTAAACAATAAAAATATGTTCCATT
TCAAATTTGTTGAACACCCTTATCTTTATTATGGGAAATCTATTAAAAGTTTGCATCTTT
GGGTTGTTCTTGTTCTTCACCTAAAAACCAAACAAGAGAGATCATTTATGTAAATTTAAA
ATAACCTTTGACAAATAATTAAAAACTCACGCCCCTTTAATATAAATCTTTGACATCTTC
GGCTATCTCTCCTTCTTCCTGGGAAAAAACATGTTTGTTTCCAATAACAACAATCCTTCA
GGGGACATAAACATGATGATCGGCAATGTTACATCAAACGGAAATCTACGGCCCCACCAA
ATCAACACATTAATCCCTAAACAACTTAATAACACATTGCGAACGTTTTTAAGGTACGGG
GGGGGTTTTTCTTTTCACCAGTGAAACGGGCAACAGCTGATTGCCCTTCACCGCCGGGCC
CTAAAAAAGTTGCACCAAGCGGTCCACCCTGGTTTGCCCCACCAGGCAAAAATCCGGTTT
GATGGTGGTTCCAAA
   
Sequence Name 50-K116054-SALK_095404C-R210
GenBank Accession LN485513 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K116054-SALK_095404C-R210
GCTGTAATCTCCGTTTGATGTAACATCGCCGATCATCATGTTTCTGTCCCGTGAAGGATT
GTTGTTATCGGAAACAAACATGTTTTCTCCAAGGAAGAAGGAGAGATAGCAGAAGATGTC
AAAGATTAATATAGAAGGCGCGTGAGTCTTTAATTATTTGTCAAAGCTTATCTTAAATTT
ACATAAATGATCTCTCTTGTTTGATTATTAGATGAAGAACAAGAACAACCCAAAGATGCT
AACTTTTAATAGATCTCCAATAATAAAGATAAGAGTGTTCAACAAATATGAAATGGAACA
TATTTTTATTGTTTAAAAAAAAAAAGGTTCTAGAAATATTTAAAAATCTTCTCCTTTGAG
CCGGGGAAACTGATTCTTTCAATCAAAAAAAAAAAAATGGCTCAAATGGAGGGGTTTTTT
TTTT

Last Updated on 06.12.2018 16:11