Sequences from confirmation amplicon


Confirmation sequences for line SALK_095484c and hit At1g77590 are shown.
Line ID SALK_095484c
Locus At1g77590 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:29149255
Primers used for PCR 39oh - LBb3, show primer details
   
Sequence Name 22-K115143-SALK_095484C-39oh
GenBank Accession LN484920 [GenBank]
Sequencing primer gene specific primer, 39oh: 5'-CTGCTGTGACTAATCCTGACTCTG-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K115143-SALK_095484C-39oh
TCAACTTCTCCATCGTGATTGTTTAGCCGCCTGCATAACACAGATGTTATAAGGAAATGA
CTGGGGGACTCAAATAGCTTGGTAATGAGTAGCAATGAACATACCTTCACAAGGGACGCA
TACACTTCTTTCACGGCTTGCTCTTTCGTGCACAGTTCTTCGAAGTTGGCAAAGTCTATT
CCTTGCTTTGAAGCCCAACCTTCAACTGTATGTTGGGACGCGACCACAAGAGCCACACAG
TAACTGTAGAACGAATCAGCATGAACCATTATGTTTTCAACATAGGGACTTATACTTAGA
GCAGCTTCAACCTGCGAAAAAAAAGAATGTTTTAAGAAGCTTCTCCGGAGAATTGAGTGA
TGAAGACAAAAAAGGGTATTCGGATTGAGAAAACTATACTTTGCCCAAGGAGACATATTC
TCCATGCTGAAGTTTAACGATATCCTTTTTTCGGTCTATTATCTCGAGGCAGCCATCAGG
GTGAAATCGTCCTATGTCTCCTGTGTAGAACCACCTCATTCCCTTTTCATCAACCTTTGA
ACATTCAAGTGTATAAAACGGTTAGAGGAAGAACCAAACCAACCAATTCTTTTAACATTG
CCGAAACCAATAATGTTGATGTAAACAAATTGACGCTNAGACAACTTAATAACACATTGC
GGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGA
TTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTNTGCCCC
AGCAGGCGAAAATCCTGTTTGATGGTGGTTC
   
Sequence Name 06-K115142-SALK_095484C-R210
GenBank Accession LN484436 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K115142-SALK_095484C-R210
CATTTGTTTCATCAACATTATTGGTTTCGGCATGTTAAAAGAATTGGTTGGTTTGGTTCT
TCCTCTAACCGTTTTATACACTTGAATGTTCAAAGGTTGATGAAAAGGGAATGAGGTGGT
TCTACACAGGAGACATAGGACGATTTCACCCTGATGGCTGCCTCGAGATAATAGACCGAA
AAAAGGATATCGTTAAACTTCAGCATGGAGAATATGTCTCCTTGGGCAAAGTATAGTTTT
CTCAATCCGAATACCCTTTTTTGTCTTCATCACTCAATTCTCCGGAGAAGCTTCTTAAAA
CATTCTTTTTTTTCGCAGGTTGAAGCTGCTCTAAGTATAAGTCCCTATGTTGAAAACATA
ATGGTTCATGCTGATTCGTTCTACAGTTACTGTGTGGCTCTTGTGGTCGCGTCCCAACAT
ACAGTTGAAGGTTGGGCTTCAAAGCAAGGAATAGACTTTGCCAACTTCGAAGAACTGTGC
ACGAAAGAGCAAGCCGTGAAAGAAGTGTATGCGTCCCTTGTGAAGGTATGTTCATTGCTA
CTCATTACCAAGCTATTTGAGTCCCCCAGTCATTTCCTTATAACATCTGTGTTATGCAGG
CGGCTAAACAATCACGATAGGAGAAGTTTGAGATACCAGCAAAGATCAAATTATTGGCAT
CTCCNTGGACGC

Last Updated on 06.12.2018 16:11