Sequences from confirmation amplicon


Confirmation sequences for line SALK_096470c and hit At1g04300 are shown.
Line ID SALK_096470c
Locus At1g04300 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:1148873
Primers used for PCR 91by - LBb3, show primer details
   
Sequence Name 25-K108444-SALK_096470C-91by
GenBank Accession LN484995 [GenBank]
Sequencing primer gene specific primer, 91by: 5'-TTCCTCAGTTCAAACAACAACATC-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K108444-SALK_096470C-91by
TCGTCAGGTCAGATCCATCCCTACACAACCAACAAACATACACTCCTCAACCCTATAAAA
ACGCTATAGTCGGTAACTCTCTTGGTTCATCATCATCTAGTTTCAACCACCACCCAAGCT
CTCATGGAGTTGTCCCGACCACATTGCCATCATCATCTTACTCGCAAGCACCAACATCAT
CTTATCAGTCAAGCTTCCCTTACAGTCAAGATGGGCTCTTGTGGACAGGAAGAAGTCCCA
GTTCTGTAAACATGGGCATGTACAACAACACTTACTCACCCGCCGTTACTAGCAACAGAT
CGCTGAACCACATGGACGTTCAGATAGCGCAGCAACAAGCACAAAGCATGATGACGGATG
AGTTCCCTCACCTAGACATCATCAATGATCTGCTGGAAGACGAACAGTGCAGCAACATGG
TGTATAACGGAAGCATATTCAATCCACAACCGCAAGTGTTCAACGGGCAGTACTCATCAT
ACCATGGTGAGTTATTGTCTGGTGGCAGGACCAGGAGTTTCGGAGAGGAAGGATTGCATT
ACATGGCACGTGGACCATATGGTACAGACGGAATGATGCCGAGACAGTGGCAAATGACGA
ACATGGACTTGTCGTTGCCAGCAATGAGGAGCAATGGAATGGAAGATGGAACATCATCAG
CAGCTAATTACCATCACAGTTACTTTGGTTTGGACGCTTCGAATCCAAGTTTATTGACAA
CTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAG
   
Sequence Name 09-K108443-SALK_096470C-R210
GenBank Accession LN484513 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K108443-SALK_096470C-R210
CTTGGATCGAAGCGTCCAACCAAAGTAACTGTGATGGTAATTAGCTGCTGATGATGTTCC
ATCTTCCATTCCATTGCTCCTCATTGCTGGCAACGACAAGTCCATGTTCGTCATTTGCCA
CTGTCTCGGCATCATTCCGTCTGTACCATATGGTCCACGTGCCATGTAATGCAATCCTTC
CTCTCCGAAACTCCTGGTCCTGCCACCAGACAATAACTCACCATGGTATGATGAGTACTG
CCCGTTGAACACTTGCGGTTGTGGATTGAATATGCTTCCGTTATACACCATGTTGCTGCA
CTGTTCGTCTTCCAGCAGATCATTGATGATGTCTAGGTGAGGGAACTCATCCGTCATCAT
GCTTTGTGCTTGTTGCTGCGCTATCTGAACGTCCATGTGGTTCAGCGATCTGTTGCTAGT
AACGGCGGGTGAGTAAGTGTTGTTGTACATGCCCATGTTTACAGAACTGGGACTTCTTCC
TGTCCACAAGAGCCCATCTTGACTGTAAGGGAAGCTTGACTGATAAGATGATGTTGGTGC
TTGCGAGTAAGATGATGATGGCAATGTGGTCGGGACAACTCCATGAGAGCTTGGGTGGTG
GTTGAAACTAGATGATGATGAACCAAGAGAGTTACCGACTATAGCGTTTTTATAGGATTG
AGGAGTGTATGTTTGTTGGTTGTGTAGGG

Last Updated on 06.12.2018 16:11