Sequences from confirmation amplicon


Confirmation sequences for line SALK_096484c and hit At3g44910 are shown.
Line ID SALK_096484c
Locus At3g44910 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:16392610
Primers used for PCR 78hw - LBb3, show primer details
   
Sequence Name 30-K112874-SALK_096484C-78hw
GenBank Accession LN485130 [GenBank]
Sequencing primer gene specific primer, 78hw: 5'-TTCTCTACGAATCAACATACGACG-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K112874-SALK_096484C-78hw
ATCATATCATTTCGTCATGATCTTCACATATTTATATGGTTTGTTTGTTTTTGCAGTATA
CATCGCAAGCAACATATACATTCTTGATCACTTGTGCTCTAATAAACTCTGGAATTATCC
CCACAGCCTTGGCCGGACTATATGACCCGAAGCGGAAGTACGTTGGTTACCAAAAAAAGA
ACATCATGAATCTAAAACCAGATTCAGATCTCCGGATCTTGACTTGTATACATAGACCTG
AGAACATCTCTGCGGCCATTTCATTTCTCCAATTTTTGCCCTCCACCATTGTGGTCACAG
TCCTCCATCTCGTTAAGCTTGTTGGCAAAACCGTTCCCGTCCTAATCTCACACAACAAGC
AAATAAATCGTGTAGTAACTAACTCCTACATCCATACCGCCAACCTTGCCTTCAGCCAGT
TGGAGTCAGTGACCATGACCATGTTCACTGCCATCACGCACGAGAACTTAATGCACGATG
AAATTTGCAAGGTCGCTTTGGAACAAGCCACATCGATNATTATCGTCCCCTCGGGGAGGA
AATGGACCGTAGATGGGGCGTTTGAGTCAGAGGACGAAGCTATAAGACGCTTAGACAACT
TAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGA
CGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCA
CGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCGAATCGGGCAAA
   
Sequence Name 22-K112873-SALK_096484C-R210
GenBank Accession LN484916 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K112873-SALK_096484C-R210
TCTAAGCTTCGTCCTCTGACTCAACGCCCCATCTACGGTCCATTTCCTCCCCGAGGGCAC
TAAAAGAATCGATGTGGCTTGTTCCAAAGCGACCTTGCACCTTTCATCGTGCATTAAGTT
CTCGTGCGTGATGGCAGTGAACATGGTCATGGTCACTGACTCCAACTGGCTGAAGGCAAG
GTTGGCGGTATGGATGTAGGAGTTAGTTACTACACGATTTATTTGCTTGTTGTGTGAGAT
TAGGACGGGAACGGTTTTGCCAACAAGCTTAACGAGATGGAGGACTGTGACCACAATGGT
GGAGGGCAAAAATTGGAGAAATGAAATGGCCGCAGAGATGTTCTCAGGTCTATGTATACA
AGTCAAGATCCGGAGATCTGAATCTGGTTTTAGATTCATGATGTTCTTTTTTTGGTAACC
AACGTACTTCCGCTTCGGGTCATATAGTCCGGCCAAGGCTGTGGGGATAATTCCAGAGTT
TATTAGAGCACAAGTGATCAAGAATGTATATGTTGCTTGCGATATATACTGCAAAAACAA
ACAAACCATATAAATATGTGAAGATCATGACGAAATGGATTATGGATTGGTAGGGAAGTT
ACCGAATCGTCGTATGTTGATTCGTAGAGAAA

Last Updated on 06.12.2018 16:11