Sequences from confirmation amplicon


Confirmation sequences for line SALK_097767c and hit At3g07800 are shown.
Line ID SALK_097767c
Locus At3g07800 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:2490047
Primers used for PCR CG72 - R212, show primer details
   
Sequence Name 57-K108702-SALK_097767C-CG72
GenBank Accession LN485658 [GenBank]
Sequencing primer gene specific primer, CG72: 5'-ACAGCAGCATGAAGTAGACGTAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K108702-SALK_097767C-CG72
AGATTCCAACAGTGGAGACACCTCTAGCATCAATAGTTTGCTCCCTAGAGATACCTTTCA
GTGATGAAGGTGGATCAAGAAAACAGTGCTTCTTAAACCAGAGCAATGCAACAATGGAAG
AATGCGAATCCACAAGTATCAAATCCAGGATCTGATGAAAGAAATGCTTCTGTCACAAAA
CAACCTCTCATACGCAACATTGTAAGTCCAACTCGAAAAACTAAGTGGTCTTGGAAAATG
AAGAGTATGAAACATGGACCAGAGGCTGTAGAGAAAGCATCAAACTTATATTTATATACA
ATGAACATCACAAGTGTATTTCAAAGACAACATTAAGGATAAACCAGACCAGTAAAGAAA
CCAAACAACCAAAAAAGAAAGAAAACTTCACCATAACATATGTTGCTTGTTCCATATCAT
TTATAGTTAGATTGTAGCAGCAACACAGGATTCAGCTCTAGCCTTGTCAGAATCTTCCAA
GACTTTCTTAGAGGCTTTAATAACAATATGATTAGTGATGTAATGCTTGCTAAGACAACT
GATAGTTTAAACTGAAGGCGGGAAACGACAATCTGATCATGAGCGGAGAATTAAGGGAGT
CACGTTATGACCCCCGCCGATGACGCGGGACAAGCCGTTTTACGTTTGGAACTGACAGAA
CCGCAACGTTGAAGGAGCCACTCAGCCGCGGGTTTCTGGAGTTTAATGAGCTAAGCACAT
ACGTCAGAAACCATTATTGCGCGTTCAAAAGTCGCCTA
   
Sequence Name 49-K108701-SALK_097767C-R214
GenBank Accession LN485474 [GenBank]
Sequencing primer gene specific primer, R214: 5'-AACGTTGCGGTTCTGTCAGTTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K108701-SALK_097767C-R214
GCGGGGGTCATAACGTGACTCCCTTAATTCTCCGCTCATGATCAGATTGTCGTTTCCCGC
CTTCAGTTTAAACTATCAGTTGTCTTAGCAAGCATTACATCACTAATCATATTGTTATTA
AAGCCTCTAAGAAAGTCTTGGAAGATTCTGACAAGGCTAGAGCTGAATCCTGTGTTGCTG
CTACAATCTAACTATAAATGATATGGAACAAGCAACATATGTTATGGTGAAGTTTTCTTT
CTTTTTTGGTTGTTTGGTTTCTTTACTGGTCTGGTTTATCCTTAATGTTGTCTTTGAAAT
ACACTTGTGATGTTCATTGTATATAAATATAAGTTTGATGCTTTCTCTACAGCCTCTGGT
CCATGTTTCATACTCTTCATTTTCCAAGACCACTTAGTTTTTCGAGTTGGACTTACAATG
TTGCGTATGAGAGGTTGTTTTGTGACAGAAGCATTTCTTTCATCAGATCCTGGATTTGAT
ACTTGTGGATTCGCATTCTTCCATTGTTGCATTGCTCTGGTTTAAGAAGCACTGTTTTCT
TGATCCACCTTCATCACTGAAAGGTATCTCTAGGGAGCAAACTATTGATGCTAGAGGTGT
CTCCACTGTTGTGAATCTCGAATCACTCTTTGGACCGAGACCATAGATCTTGACATCTAC
GTCTACTTCATGCTGCTG
Primers used for PCR CG71 - LBb3, show primer details
   
Sequence Name 58-K108702-SALK_097767C-CG71
GenBank Accession LN485683 [GenBank]
Sequencing primer gene specific primer, CG71: 5'-TGATGATGGTAAAATTGTGATCGT-3'
Sequence (A. th genome BLAST matches underlined) 
>58-K108702-SALK_097767C-CG71
CTTCATCCTAGTACAAAGCTGTGATTTTGATATTTTACTTCAATGTTCAACAATTTTGTT
GACTGTAAAATGTGGAATTGATCTCTCTATGTGCAGGAGGAGTTTTGGGGCTGTACTTGA
CATTATACCAATAGCTGATTCTGTGACTAAGCTAACTGCAAGGTGTGAGGTCTGTGGACA
TAAAGCTTTCTTCACTTTAAGAAAGAATTGTGACACCAGAACTGAGCTTATTGGTGGAGC
TGATGTCTATAACAAATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAAT
GTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCG
CCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAAT
CCTGTTTGATGGTGGTTCCGAAATCGGCAAAA
   
Sequence Name 50-K108701-SALK_097767C-R210
GenBank Accession LN485500 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K108701-SALK_097767C-R210
GTCATTTGTTTAGACATCAGCTCCACCAATAAGCTCAGTTCTGGTGTCACAATTCTTTCT
TAAAGTGAAGAAAGCTTTATGTCCACAGACCTCACACCTTGCAGTTAGCTTAGTCACAGA
ATCAGCTATTGGTATAATGTCAAGTACAGCCCCAAAACTCCTCCTGCACATAGAGAGATC
AATTCCACATTTTACAGTCAACAAAATTGTTAAACATTGAAGTAAAATATCAAAATCACA
GCTTTGGTACTAGGATGAAGCACCTTAAATAGTCACCATCTAGGCCAGCAACGATCACAA
TTTTACCATCATC

Last Updated on 06.12.2018 16:11