Sequences from confirmation amplicon


Confirmation sequences for line SALK_097868c and hit At1g08980 are shown.
Line ID SALK_097868c
Locus At1g08980 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:2884413
Primers used for PCR BL17 - R204, show primer details
   
Sequence Name 35-K104740-SALK_097868C-BL17
GenBank Accession LN485213 [GenBank]
Sequencing primer gene specific primer, BL17: 5'-CCTGTAAGCCATTTCATCCATAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K104740-SALK_097868C-BL17
TGAACTACTGGAGCTGTAGAAGTAGCTGCCGAGTGTCTTTTTAACCAATCCGGGTTACCA
AAACCGGTAACGCGTCCTTCCACATCAAAACTGATCAAACCAAGCAATAAGCTCAAAAAT
TGATTGACAAGAAAGTATAGAAGAAACTCTGTAAGTAGTTGGATATCTTACATGTCTTTA
ATGGCGAAAGTAAGACCCTGTAGAGAAGGAGGCGACGAGGAAGTAGAAGTTGGAGAGATT
GTAACTTTCTCAATGAAAGCTCCAAAATCATTATTGGTCGCCATTGATGCAGCTCCCTTT
CTGAGAAGGTAGATGACGACCAAGATATATTGACAAATTGACGCTTAGACAACTTAATAA
CACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCA
ACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACG
   
Sequence Name 93-K104792-SALK_097868C-R210
GenBank Accession LN486349 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>93-K104792-SALK_097868C-R210
CGTCATTTGTCATATATCTTGGTCGTCATCTACCTTCTCAGAAAGGGAGCTCCCTTAATG
GCGACCAATAATGATTTTGGAGCTTTCATTGAGAAAGTTACAATCTCTCCAACTTCTACT
TCCTCGTCGCCTCCTTCTCTACAGGGTCTTACTTTCGCCATTAAAGACATGTAAGATATC
CAACTACTTACAGAGTTTCTTCTATACTTTCTTGTCAATCAATTTTTGAGCTTATTGCTT
GGTTTGATCAGTTTTGATGTGGAAGGACGCGTTACCGGTTTTGGTAACCCGGATTGGTTA
AGGACACACTCGGCAGCTACTTCTACAGCTCCAGTAGTTTCATCTCTCTTAGAAGCTGGT
GCCACAGCTTTGGGTATTACCATTATGGATGAAATGGCTTACAGG
Primers used for PCR BL18 - LBb3, show primer details
   
Sequence Name 79-K121396-SALK_097868C-BL18
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, BL18: 5'-TAGCAAGAAACTGTGAGTCTTCCA-3'
Sequence (A. th genome BLAST matches underlined) 
>79-K121396-SALK_097868C-BL18
GGGTTTGTTTCGTTTTCGAATAATGGTAACCTTTGTATAACTTATTTCAGTATCGTCTCA
NTTTGGTACTATGTCAGTTTTGGTATTCTTATCTTATTGAAGTAAAAATGTGATAGTGTA
TTCGCTTTTCTCCCTGCAGCGTTCTAGTCTCGTCCAGGTGAAAGGAGGGAGACCTTAGAC
AAGAAAATGAAAAGAACATATCAGAATTGTCAAATTGGGTGTTGTAGAAGAATCTAAAGT
GTTTATGTCTATCGATCCTTTTCTAAACGAGAAATTTAGGGATGAACCAGAAAGAAGAAT
GTATAAACTGTAAGAAACAAGTGTGCTTTTGACAAAAGTTTTTTTATTTGAATGTTAATT
TCTGTGTCTTTATCACACATATTAAGTTATTATCCACAAAATGTATCTCATTGGATATTT
AAAACAAAAGGACAATAATATTAAGGAAAAGACAAAATAGAAAATGACTTGGCCACTGTA
GCCGCGTTAATAATGTGATAGCTTTTATCTACAAGAAGCGTTAACACATTGCGGTGTAAA
CAAATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGG
TTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAG
AGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGG
TGGTTC
   
Sequence Name 41-K121395w-SALK_097868C-R210
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K121395w-SALK_097868C-R210
AACGCTTTCTTGTAGATAAAAGCTATCACATTATTAACGCGGCTACAGTGGCCAAGTCAT
TTTCTATTTTGTCTTTTCCTTAATATTATTGTCCTTTTGTTTTAAATATCCAATGAGATA
CATTTTGTGGATAATAACTTAATATGTGTGATAAAGACACAGAAATTAACATTCAAATAA
AAAAACTTTTGTCAAAAGCACACTTGTTTCTTACAGTTTATACATTCTTCTTTCTGGTTC
ATCCCTAAATTTCTCGTTTAGAAAAGGATCGATAGACATAAACACTTTAGATTCTTCTAC
AACACCCAATTTGACAATTCTGATATGTTCTTTTCATTTTCTTGTCTAAGGTCTCCCTCC
TTTCACCTGGACGAGACTAGAACGCTGCAGGGAGAAAAGCGAATACACTATCACATTTTT
ACTTCAATAAGATAAGAATACCAAAACTGACATAGTACCAAACTGAGACGATACTGAAAT
AAGTTATACAAAGGTTACCATTATTCGAAAACAGAAACAAAACCCCAAGACTACAAGGTA
GGCCTAAAAGAAGCTGGAAGACTC

Last Updated on 06.12.2018 16:11