Sequences from confirmation amplicon


Confirmation sequences for line SALK_098531c and hit At5g54630 are shown.
Line ID SALK_098531c
Locus At5g54630 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:22193938
Primers used for PCR 77xe - LBb3, show primer details
   
Sequence Name 27-K119050-SALK_098531C-77xe
GenBank Accession LN485075 [GenBank]
Sequencing primer gene specific primer, 77xe: 5'-AACACTTGTTGACCCGTTTATACC-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K119050-SALK_098531C-77xe
ACTAAGTGCTCGTTCCGTCTGCGAGACATCTTGGATGCTTCTTCTGAAGTTTGCTTGCTC
TGATTTTAACTGTTTCTCTGTATTCCTCGAATCTAGCTAATGTCTTCTGCATGTTGTGTA
CTTTTAAAACTCTATCGATTCTTCCACATTGGTTCTCTGACTTTAACCAGCTTGTTCTGC
AGATTATCTCCACTATTTTTCTTGATGAATCTCCTTCTACAAGCTCCGTTACTGCAATTT
AGATGAAAGTTATATAAATTTGAGAAACTTTGAATCAAACTGCATAATCAAAATCGGAAA
TTTAGAGTAAATGTTGTTAAAGTTTAACATTTTAGCTTTGAATTGTTAATTGCAAAACTC
TGTTTTTTTCAAGACTCTGTTTTTTTCTTTATAAAAGTTGCGATTTTTATAACCTAACTT
TTGTTACTATGAAGCCTAAATTGCATAATTTGTTAAATTTACTTACCGGCATGTTTAGAG
AGATGATGAGCTTCAGCAGCTTCAAGCTTATTGAACTGTTCACCACATTTATGACAAGAA
ACAGAGGAATTGTTGCCTCCATTAATGGTGGATTCTCTGTTCATCTCCAATGAAACCCTT
CTGTTTGTATGAAACCCTAACCCTTCTCCTCCTCCTCCTCCTCCTCGATCTCTCTCCGAC
AAAGCAAACGAACCCTTTCTTGTTTGGCTCCTGTAAGAAGCTGAATGGTTAGATAATGCA
TCGGTGTTCCTGGTCTAAGCATCCCTACATAAATCCTAGACCGACCATTTCTTCCACGCC
ACCTCCGCCGGAATCCGCTGCTCCGACAGGT
   
Sequence Name 11-K119049-SALK_098531C-R210
GenBank Accession LN484600 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K119049-SALK_098531C-R210
CATTTGTCATTTGGAAGCATGAGTTTCTAAATCCTATAACTCATGAAGTTATTCTTAGTA
ACTCAACTTGTGAGCTTAAGATCACTGGTGTCGGAGACATGGCTTCACCTGTCGGAGCAG
CGGATTCCGGCGGAGGTGGCGGTGGAGGAAATGGTCGGTCTACGACTTATGTAGGGATGC
TTAGACCAGGAACACCGATGCATTATCTTAACCATTCAGCTTCTTACAGGAGCCAAACAA
GAAAGGGTTCGTTTGCTTTGTCGGAGAGAGATCGAGGAGGAGGAGGAGGAGGAGAAGGGT
TAGGGTTTCATACAAACAGAAGGGTTTCATTGGAGATGAACAGAGAATCCACCATTAATG
GAGGCAACAATTCCTCTGTTTCTTGTCATAAATGTGGTGAACAGTTCAATAAGCTTGAAG
CTGCTGAAGCTCATCATCTCTCTAAACATGCCGGTAAGTAAATTTAACAAATTATGCAAT
TTAGGCTTCATAGTAACAAAAGTTAGGTTATAAAAATCGCAACTTTTATTAAGAAAAAAA
CATAGTC

Last Updated on 06.12.2018 16:11