Sequences from confirmation amplicon


Confirmation sequences for line SALK_098534c and hit At3g60840 are shown.
Line ID SALK_098534c
Locus At3g60840 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:22477945
Primers used for PCR 15jg - LBb3, show primer details
   
Sequence Name 29-K113259-SALK_098534C-15jg
GenBank Accession LN485109 [GenBank]
Sequencing primer gene specific primer, 15jg: 5'-TTCTTTCACTTGTCTCATCAAACG-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K113259-SALK_098534C-15jg
GCAGTAATCGGATACATGGTGTAGCAGCTTTCTAAGAGAGGAGACCAGTTTTTTCACGGT
TTAGCAAAAACCGGCCCTAACCTCTTCAAACGAATACTCAACAATAACGTCTTCCTCATC
CATTCTCTTCTCCACAGCAGGAGTGAACGGCGTTGTTGCCTCTGTCATAGCGACTGAAGC
TGCAGGAGTTGTTGGAACAGCTCTTTTCTTTTCTTCGTTGGTTTTAGGTGTCAAGATTCT
CTCCTTTGTTGTGTAAGCATCCTTGTGATGATCTTCTGGGGAATTCAAGATATTGGAAGT
GACAGGTGAAAGAGGCTTCCTAAGCATTGGAGATCTCGCAGCTTTTCCTGCAACCGGAAC
GTTCTTTGATCTGATACGACCAGGAAGCTCTGAGTTTCTCCTCCCTACACATATTGACAT
AATTCTCTTAAACAAATATTGTGGACAAATTGACGCTTAGACAACTTAATAACACATTGC
GGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGA
TTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCC
AGCAGGCGAAAATCCTGTTTGATGGTGGTTCCGAATCGGGCAAAA
   
Sequence Name 12-K113258w-SALK_098534C-R210
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K113258w-SALK_098534C-R210
GTCATTTGTCCCATATTTGTTTAGAGAATTATGTCAATATGTGTAGGGAGGAGAAACTCT
GAGCTTCCTGGTCGTATCAGATCAAAGAACGTTCCGGTTGCAGGAAAAGCTGCGAGATCT
CCAATGCTTAGGAAGCCTCTTTCACCTGTCACTTCCAATATCTTGAATTCCCCAGAAGAT
CATCACAAGGATGCTTACACAACAAAGGAGAGAATCTTGACACCTAAAACCAACGAAGAA
AAGAAAAGAGCTGTTCCAACAACTCCTGCAGCTTCAGTCGCTATGACAGAGGCAACAACG
CCGTTCACTCCTGCTGTGGAGAAGAGAATGGATGAGGAAGACGTTATTGTTGAGTATTCG
TTTGAAGAGGTTAGGGCCGGTTTTTGCTAAACCGTGAAAAAACTGGTCTCCTCTCTTTAG
AAAGCTGCTACACCATGTATCCGATTTACTGCTTTTTCTAGAAATTTCAAGTCAGATACT
TCGTTTGATGAGACAGTGGAAAAGA

Last Updated on 06.12.2018 16:11