Sequences from confirmation amplicon


Confirmation sequences for line SALK_099019c and hit At1g55920 are shown.
Line ID SALK_099019c
Locus At1g55920 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:20912322
Primers used for PCR 62cs - LBb3, show primer details
   
Sequence Name 71-K110924-SALK_099019C-62cs
GenBank Accession LN486027 [GenBank]
Sequencing primer gene specific primer, 62cs: 5'-GCTTGGTAGGTTTAAATTGCTGAG-3'
Sequence (A. th genome BLAST matches underlined) 
>71-K110924-SALK_099019C-62cs
CTAGCAACTCTAAGATCGATGAGATGTGATCGAAGCGTAGTAGTAGTTTGATCAATGGGT
TCTTGTTTACATCGGATTTGGCTTCTTCAAGCATCTTGATCCAGACATCATCGTCATCTT
CGATTTGGGTGTGGTGAATCTTCCGGTTTACAGAGAAACCGGGTCGAAAGAAATTCTTGA
TGCAACAGAACCGGGAATCATCGTCTTGGGTATTACCGGTTCGGCATGTGTCTATGCATG
TTGCCATGGTTTATGATTTGGAAGAAGAAAGAGTTTGAGATAAAGAAGAGAAGAGAGAAT
GGTTGTTGAGTACAAATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAAT
GTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCG
CCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAAT
CCTGTTTGATGGTGGTTCCGAATCGGGCAAAA
   
Sequence Name 67-K110923-SALK_099019C-R210
GenBank Accession LN485925 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>67-K110923-SALK_099019C-R210
GTCATTTGTCTCACACCATTCTCTCTTCTCTTCTTTATCTCAAACTCTTTCTTCTTCCAA
TCATAAACCATGGCAACATGCATAGACACATGCCGAACCGGTAATACCCAAGACGATGAT
TCCCGGTTCTGTTGCATCAAGAATTTCTTTCGACCCGGTTTCTCTGTAAACCGGAAGATT
CACCACACCCAAATCGAAGATGACGATGATGTCTGGATCAAGATGCTTGAAGAAGCCAAA
TCCGATGTTAAACAAGAACCCATTTTATCAAACTACTACTACGCTTCGATCACATCTCAT
CGATCTTTAGAGTCTGCTTTAGCTCACATCCTCTCCGTAAAGCTCAGCAATTTAAACCTA
CCAAG

Last Updated on 06.12.2018 16:11