Sequences from confirmation amplicon


Confirmation sequences for line SALK_099193c and hit At4g10230 are shown.
Line ID SALK_099193c
Locus At4g10230 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:6366995
Primers used for PCR 39ux - LBb3, show primer details
   
Sequence Name 33-K117668-SALK_099193C-R210
GenBank Accession LN485190 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K117668-SALK_099193C-R210
TTTGTTCAGATGCTCGATATTGATGATCATGTTGGTTTGTTTGTGCTCTTTTTTCCAAAA
TTCTTTTTCTCTCATTTTCAACATATGCACGAGAGGATGGATCACTTATAGAATTCAAAT
CTGCAAATAATATTTTATTCTCTTCTTTCATTCTAGCTACCTCGATCTTCTGCCTTTGAA
TTTCATTTCTTTCAGAAGTACCTTTAGTAATAGCTTTGATAAGTTTATCATTTTGCTCCA
AAAGTTGTTTAAACTGTTCTTCAGATTGTTGTTTTCGCTTTGCTTTTTTCAAACCCATAG
GCCTTGAAGATAAACTAAAATTGGCATCTTCACTATCCATATTTAGATCAATTGAATTCA
TACCAGGGGAAGGTGATGACTCGGTATTGATTGAAAATGATGAAGATGACGTAACATCAC
GACCTTCTCCTTGGAATGCAGGGGGTGTCTTCATGNTATCATTA
   
Sequence Name 70-K117669-SALK_099193C-39ux
GenBank Accession LN486013 [GenBank]
Sequencing primer gene specific primer, 39ux: 5'-TAATGATAACATGAAGACACCCCC-3'
Sequence (A. th genome BLAST matches underlined) 
>70-K117669-SALK_099193C-39ux
TCATCTTCTCATTTTCATCAATACCGAGTCATCACCTTCCCCTGGTATGAATTCAATTGA
TCTAAATATGGATAGTGAAGATGCCAATTTTAGTTTATCTTCAAGGCCTATGGGTTTGAA
AAAAGCAAAGCGAAAACAACAATCTGAAGAACAGTTTAAACAACTTTTGGAGCAAAATGA
TAAACTTATCAAAGCTATTACTAAAGGTACTTCTGAAAGAAATGAAATTCAAAGGCAGAA
GATCGAGGTAGCTAGAATGAAAGAAGAGAATAAAATATTATTTGCAGATTTGAATTCTAT
AAGTGATCCATCCTCTCGTGCATATGTTGAAAATGAGAGAAAAAGAATTTTGGAAAAAAG
AGCACAAACAAACCAACATGATCATCAATATCGAGCATCTGTAACAAATTGACGCTTAGA
CAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAG
TGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCG
GTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCGA

Last Updated on 06.12.2018 16:11