Sequences from confirmation amplicon


Confirmation sequences for line SALK_100402 and hit At5g24450 are shown.
Line ID SALK_100402
Locus At5g24450 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:8352670
Primers used for PCR 21rb - LBb3, show primer details
   
Sequence Name 79-K116055-SALK_100402C-21rb
GenBank Accession LN486210 [GenBank]
Sequencing primer gene specific primer, 21rb: 5'-TTGACAATTTGAGACTCCGTGTAG-3'
Sequence (A. th genome BLAST matches underlined) 
>79-K116055-SALK_100402C-21rb
TTTCGAGACGTTTTTAATCTATTCAGAAGAGTTTGAGAGGTCTGAAAAGACACGTATTCA
GAAAGATGCACTGCAACCAAGTCAACGGAATCACCAGGAAACAACTAAAGGTAAGATAGA
CAAAGCAATTTCAGTTCTTTTCATCTTTACATGTTAGGCAGCCTAGTTAGTGATTGTTGA
CATGCATGGAGCCAAGTGCTTCTTTTTCAAATCTCAATGGTCTCAAAATTGTGTTAAAGC
ATGGTGAACTTGACTGGAGTTAATTTGTTGGACATATTGTACGACGAATTTGTAAGTAAA
TATATAAGGGTGCACAATCACATGTGCCAGCCAAAATCGAATGAGGCAGGCATTTTATAT
TGTCATACATCTCATGCTCTGCTTATTATCATTTGTCATCAAACACATTCTCCTTAAAAG
AAGTATGGATTAACAGTTGTTGGTGTAAAATTATCCGCAGATATGAAAAAGTGTAAAAAC
ACAAACAAGGAGAAAGATGATGATGTTAATGCGGATGAGGATTCTGAAGACTTGGATGAT
ATCAATTTACTACTTAATAACACACAGGATATATTGTGGTGTAAACAAATTGACGCTTAG
ACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCA
GTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGC
GGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCG
   
Sequence Name 52-K116054-SALK_100402C-R210
GenBank Accession LN485564 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K116054-SALK_100402C-R210
TCATTTGTTAACCACAATATATCCTGTGTGTTATTAAGTAGTAAATTGATATCATCCAAG
TCTTCAGAATCCTCATCCGCATTAACATCATCATCTTTCTCCTTGTTTGTGTTTTTACAC
TTTTTCATATCTGCGGATAATTTTACACCAACAACTGTTAATCCATACTTCTTTTAAGGA
GAATGTGTTTGATGACAAATGATAATAAGCAGAGCATGAGATGTATGACAATATAAAATG
CCTGCCTCATTCGATTTTGGCTGGCACATGTGATTGTGCACCCTTATATATTTACTTACA
AATTCGTCGTACAATATGTCCAACAAATTAACTCCAGTCAAGTTCACCATGCTTTAACAC
AATTTTGAGACCATTGAGATTTGAAAAAGAAGCACTTGGCTCCATGCATGTCAACAATCA
CTAACTAGGCTGCCTAACATGTAAAGATGAAAAGAACTGAAATTGCTTTGTCTATCTTAC
CTTTAGTTGTTTCCTGGTGATTCCGTTGACTTGGTTGCAGTGCATCTTTCTGAATACGTG
TCTTTTCAGACCTCTCAAACTCTTCTTGAATAGATTTAAAAACGTCTTCGAAACCTGGCT
CAGGAAACACAGATACAAACCTCACAGCTACACGGAGTCTCAAATTGTCAA

Last Updated on 06.12.2018 16:11