Sequences from confirmation amplicon


Confirmation sequences for line SALK_100861c and hit At4g15020 are shown.
Line ID SALK_100861c
Locus At4g15020 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:8576959
Primers used for PCR 29td - LBb3, show primer details
   
Sequence Name 19-K116922-SALK_100861C-29td
GenBank Accession LN484828 [GenBank]
Sequencing primer gene specific primer, 29td: 5'-AATTCTGTTAATTTCCAACCGATG-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K116922-SALK_100861C-29td
GGAGATCGGGGTATCTGCTCCACGCATGATGACCTTCGAGGGTGGATACTGAAGAATTGT
GTTGAGGAGATGGCGAAAGAGATTGATGAATGCAAAGCGATGTGGAAGAGGACTGGCTGT
TCTATTTTGGTTGAGGAACTGAACTCTGATAAAGGCTTTAAAGTTCTTAACTTTTTGGTT
TATTGCCCTGAAAAGGTAGTGTTTCTGAAATCTGTGGACGCATCTGAGGTTTTATCTTCT
GCTGATAAATTGTTTGAGCTACTTAGCGAACTGGTTGAAGAAGTTGGCAGTACCAATGTT
GTTCAAGTGATTACAAAATGTGATGATTACTACGTTGATGCTGGGAAAAGGTTGATGCTT
GTGTATCCTTCTCTATATTGGGTTCCTTGCGCTGCACATTGCATAGACCAGATGCTCGAG
GAGTTTGGGAAACTTGGCTGGATAAGTGAGACTATTGAACAGGCTCAAGCTATTGTACTG
GGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGC
CCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTT
TGATGGTGGTTCCGAATCGGGCAAA
   
Sequence Name 08-K116921-SALK_100861C-R210
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K116921-SALK_100861C-R210
AACGTTATCTTGTACTTCAGACTCACTTTAGACCTCAATA

Last Updated on 06.12.2018 16:11