Sequences from confirmation amplicon


Confirmation sequences for line SALK_101706c and hit At1g32260 are shown.
Line ID SALK_101706c
Locus At1g32260 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:11641924
Primers used for PCR 22rb - LBb3, show primer details
   
Sequence Name 80-K116055-SALK_101706C-22rb
GenBank Accession LN486221 [GenBank]
Sequencing primer gene specific primer, 22rb: 5'-TCAAGTCTCATTGATTGGTTTGAG-3'
Sequence (A. th genome BLAST matches underlined) 
>80-K116055-SALK_101706C-22rb
CGATCGCATTTCACACAGAGCGACGCAATTTCACATTCTGGAAAACCAAATTTCTCAAAT
GCGATTTCGTATCCAACGACATATTTATCAGAATCGAAACGATCTCGATTAGAAATCTAA
CCGATGAAGAAATTGACGGATTTTGGGATGTTTCGGAGTAACAAGCCGACGCTGGATCTT
CAGAGCGAGCCTATAAGTCCGTCTCAGTCCCAAGAAGTAAGCTGTTCCTAGAGTTATCTC
CCAAAACACCATCTTCTTTCTTCTTCTTCTTCTTCTTCTTCGGCTACACGATTATCGCAT
GATCGCCATTTTCGTCAGTCACTGACCTTTTGGCTGGTTAACGGTGATGCGATCCTCACC
GTCTATTTCCTTGTTCGAAGAAGTCATTGTGGGCCGTCTGTTGGCCCACTTTAACGGAGT
AGATATATGGGCCGTCTGTTTTTTCATAATTTTGTAAAGTTTACAACTTAATAACACATT
GCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCT
GATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCC
CCAGCAGGCGAAAATCCTGTTTGATGGTGGTTC
   
Sequence Name 53-K116054-SALK_101706C-R210
GenBank Accession LN485586 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K116054-SALK_101706C-R210
AAAGTGGGCCAACATTTTGACCACAATGACTTCTTCAAACAAGGAAATAGACGGTGAGGA
TCGCATCACCGTTAACCAGCCAAAAGGTCAGTGACTGACGAAAATGGCGATCATGCGATA
ATCGTGTAGCCGAATAAGAAGAAGAAGAAGAAGAAAGATGATGGTGTTTTGGGAGATAAC
TCTAGGAACAGCTTACTTCTTGGGACTGAGACGGACTTATAGGCTCGCTCTGAAGATCCA
GCGTCGGCTTGTTACTCCGAAACATCCCAAAATCCGTCAATTTCTTCATCGGTTAGATTT
CTAATCGAGATCGTTTCGATTCTGATAAATATGTCGTTGGATACAAAATCGCATTTGAGA
AATTTGGTTTTTCCAAAAAGTGAAATTGCGTCGCTCTGTTGTTGAAATTGCTGATTCGGT
TTAGGTTTTCTAATCGTTTACTCAAACCAATCAATGAGACTTGAATGTGGTTTTTTGCGT
CTCGACAAAAAAACCT

Last Updated on 06.12.2018 16:11