Sequences from confirmation amplicon


Confirmation sequences for line SALK_101859c and hit At1g21200 are shown.
Line ID SALK_101859c
Locus At1g21200 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:7421572
Primers used for PCR 43um - LBb3, show primer details
   
Sequence Name 85-K117830-SALK_101859C-43um
GenBank Accession LN486289 [GenBank]
Sequencing primer gene specific primer, 43um: 5'-GAAACATGATAACCTCTCTCAGCC-3'
Sequence (A. th genome BLAST matches underlined) 
>85-K117830-SALK_101859C-43um
CAAACTTTCTTCTACACTACTATCTATACTCGACTCATCTCCTATATAAGAAACAGCAGT
AATCAACAACTTCACCATCTTATCAGTCCATTTCACTCGTTGCCATGGAGATCCTTTAGT
TGATCTATTAGCTTCATTATGAACACCATCACCACCTTCCTCTGTGAAACTTGGTTCATC
ATCATCACTCACTGAATTCTTCTCTCTTTCAGCTTTCTGTTGCTCAGACATAGACATGTT
CTGATTCTGATGATGATCACAGGTTTGACCTGTAACCATAGTAAAGGGAAGACCTTCATG
AAGTGGTCTAGAATTAGGATTGTGTCGATGCTGCTGATTCATGGAGTCTTGGTGATGAAC
TCTCATAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCT
TTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTG
CAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCC
GAAATCGGCAAAAT
   
Sequence Name 68-K117829-SALK_101859C-R210
GenBank Accession LN485963 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>68-K117829-SALK_101859C-R210
TCACCAGACTCCATGAATCAGCAGCATCGACACAATCCTAATTCTAGACCACTTCATGAA
GGTCTTCCCTTTACTATGGTTACAGGTCAAACCTGTGATCATCATCAGAATCAGAACATG
TCTATGTCTGAGCAACAGAAAGCTGAAAGAGAGAAGAATTCAGTGAGTGATGATGATGAA
CCAAGTTTCACAGAGGAAGGTGGTGATGGTGTTCATAATGAAGCTAATAGATCAACTAAA
GGATCTCCATGGCAACGAGTGAAATGGACTGATAAGATGGTGAAGTTGTTGATTACTGCT
GTTTCTTATATAGGAGATGATTCGAGTATAGATAGTAGTAGTAGAAGAAAGTTTGCTGTT
TTGCAGAAGAAAGGGAAGTGGAAATCTGTTTCTAAAGTTATGGCTGAGAGAGGTTATCAT
GTTTCAGAAGTTGTTNATTACCGCGGTTTCGGAGAGAGGAGATGATTCGAGTATCGACGT
AGTAGTAGAAGAAAGTTTGCTGTTTTGCACAAGAAAGGGAAGTGGAAATCTGTTTCTTTT
GTTATGGCTGAG

Last Updated on 06.12.2018 16:11