Sequences from confirmation amplicon


Confirmation sequences for line SALK_101982c and hit At1g45000 are shown.
Line ID SALK_101982c
Locus At1g45000 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:17009629
Primers used for PCR 93by - LBb3, show primer details
   
Sequence Name 22-K108893-SALK_101982C-93by
GenBank Accession LN484907 [GenBank]
Sequencing primer gene specific primer, 93by: 5'-AAACAACAGGATCAACCTACCAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K108893-SALK_101982C-93by
ACAGTACAATACACTAAGCAGCTACAAAAAAAAAAAGCAAAAATCCGGAAATCAAGCATA
CTTCCCAAGGAAGGGCTCGCATAATTGTCAGCGTAGCCATATCCGGGACAACTCGAGTCC
CAAAAGAAAGCTTTCCCTTGCCCCCCTGGCTACAACACCCCCCCACATAACGGGGGCCAC
TACTTGCCTTAACATTCCCTAAAAAAAAAAAAAAAAATTCCCCCCTTTAAATGATAAAAA
ACCCAAGGGGTAGTATTCAATCAAAAACATAGGGTAGCAAGATAAAAATTTCCTTCGCTC
GTTATCAAAAGGACGTAGAACTTCCCCAAAAATCGGGCCAACGCTTTAAAAAAACTTCAA
ATCACCCCCAGTTTTGTGGGCTATTGGGGCCCGGCTATATGGAGCAGGATATATGGGGGG
GAAAACAAATGGACCCTAAAACAACTTAATAACACATGGCGAACGTTTTAAAGGAACGGG
GGGGGTTTTTCTTTTCCCCAGTGAAACGGGCAACAGCTGATTGCCCTTCACCGCCGGGCC
CTGAGAAAGTTGCACCAAGCGGCCCACGCTGGTTTGCCCCAGCAGGCAAAAATCCGGTTT
GAGGGGGGTCCCA
   
Sequence Name 06-K108892-SALK_101982C-R210
GenBank Accession LN484422 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K108892-SALK_101982C-R210
GTCATTTGTTTACCCACAATATATCCTGCTCCATATAGCCGGACCCAAATAGCCAACAAA
ACTGAGGATGATTTGAAGTCTCTTCAAAGCGTTGGCCAGATTATTGGAGAAGTACTACGT
CCTCTTGATAACGAGCGATGTAATTTTCTATCTTGCTACCTTATGTTTTTGATTGAATAC
TACACTTTGTGTTTTCTATCATCTAAAGGTGGAATTTTTTCTTCTTCTTTTTAGTGATTG
TTAAGGCAAGTAGTGGCCCTCGTTATGTGGTGGGTTGTCGTAGCAAGGTGGACAAGGAAA
AGCTTACTTCTGGAACTCGAGTTGTCCTGGATATGACTACGCTGACAATTATGCGAGCCC
TTCCTCGTGAAGTATGCTTGATTTCAGTATTTTTGCTTTTTTTTTTTGAACCTGCTTAGG
GTATTTGTACGGGTTTTAATTCAAGGGAATGTGTGGGAAGGTTAACCCTGTTGTTT

Last Updated on 06.12.2018 16:11