Sequences from confirmation amplicon


Confirmation sequences for line SALK_102687 and hit At3g11020 are shown.
Line ID SALK_102687
Locus At3g11020 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:3456517
Primers used for PCR AD04 - R204, show primer details
   
Sequence Name 54-K106282-SALK_102687C-R210
GenBank Accession LN485593 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K106282-SALK_102687C-R210
GATTTTAGATGTTAGAGAAGAGTCTTGTGGAACCAGGCCGGACAGTTGCACGGTTGGACA
TCAAGATATGAATTCTTCGCTGAATTACGATTTGCTGTTAGAGTTTGAGCAGCAGTATTG
GGGCCAAGTTTTGCAGGAGAAAGAGAAACCGAAGCAGGAAGAAGAGGAGATACAGCAACA
GCAACAGGAACAGCAACAGCAACAGCTGCAACCGGATTTGCTTACTGTTGCAGATTACGG
TTGGCCTTGGTCTAATGATATTGTAAATGATCAGACTTCTTGGGATCNCTAATGAG
   
Sequence Name 70-K106283-SALK_102687C-AD04
GenBank Accession LN485995 [GenBank]
Sequencing primer gene specific primer, AD04: 5'-CTCATTAGGATCCCAAGAAGTCTG-3'
Sequence (A. th genome BLAST matches underlined) 
>70-K106283-SALK_102687C-AD04
AGGCCACCGTAATCTGCAACAGTAAGCAAATCCGGTTGCAGCTGTTGCTGTTGCTGTTCC
TGTTGCTGTTGCTGTATCTCCTCTTCTTCCTGCTTCGGTTTCTCTTTCTCCTGCAAAACT
TGGCCCCAATACTGCTGCTCAAACTCTAACAGCAAATCGTAATTCAGCGAAGAATTCATA
TCTTGATGTCCAACCGTGCAACTGTCCGGCCTGGTTCCACAAGACTCTTCTCTAACATCT
AAAATCTGACTAAATGGATTAGATTCACAAACAACTTAATAACACATTGCGGACGTTTTT
AATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCA
CCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACG
Primers used for PCR 04au - LBb3, show primer details
   
Sequence Name 63-K119294-SALK_102687C-04au
GenBank Accession LN485834 [GenBank]
Sequencing primer gene specific primer, 04au: 5'-GGGTAAAGGAGGACCAGATAATTC-3'
Sequence (A. th genome BLAST matches underlined) 
>63-K119294-SALK_102687C-04au
GATTTGGGGTAATGGGTTGCAGAGATTCGAGAACCGAAAATAGGAACTAGACTTTGGCTT
GGTACTTTTCCTACCGCGGAAAAAGCTGCTTCCGCTTATGATGAAGCGGCTACCGCTATG
TACGGTTCATTGGCTCGTCTTAACTTCCCTCAGTCTGTTGGGTCTGAGTTTACTAGTACG
TCTAGTCAATCTGAGGTGTGTACGGTTGAAAATAAGGCGGTTGTTTGTGGTGATGTTTGT
GTGAAGCATGAAGATACTGACAAATTGACGCTTAGACAACTTAATAACACATTGCGGACG
TTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCC
CTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAG
GCGAAAATCCTGTTTGATGGTGGTTCCGAAATCGGCAAAA
   
Sequence Name 15-K119293-SALK_102687C-R210
GenBank Accession LN484711 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K119293-SALK_102687C-R210
TTTGTCGTATCTTCATGCTTCACACAACATCACCACAAACAACCNGCCTTATTTTCAACC
GTACACACCTCAGATTGACTAGACGTACTAGTAAACTCAGACCCAACAGACTGAGGGAAG
TTAAGACGAGCCAATGAACCGTACATAGCGGTAGCCGCTTCATCATAAGCGGAAGCAGCT
TTTTCCGCGGTAGGAAAAGTACCAAGCCAAAGTCTAGTTCCTATTTTCGGTTCTCGAATC
TCTGCAACCCATTTACCCCAAATCCTTTGTCTAACTCCTCTAAAACTACAGTGAGAATTA
TCTGGTCCTCCTTTACCC

Last Updated on 06.12.2018 16:11