Sequences from confirmation amplicon


Confirmation sequences for line SALK_103601c and hit At3g50720 are shown.
Line ID SALK_103601c
Locus At3g50720 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:18847658
Primers used for PCR 56aq - R204, show primer details
   
Sequence Name 06-K107313-SALK_103601C-56aq
GenBank Accession LN484420 [GenBank]
Sequencing primer gene specific primer, 56aq: 5'-CTTGCAAGAGAAAAGACTCTTGGT-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K107313-SALK_103601C-56aq
CGTTGGATGGCTCCGAGGTAATGATTCTTTCTAGACTAAATATTTGACTAGAACATATCA
TGATTAATGATTGTATGTTTTGTATCCACAGGTATGCAGCCGTGAGCCACTCCGAATTGG
GGAGAAAAAACACTACGACCAGAAAATCGATGTGTATAGCTTCGCACTGATTTTCTGGTC
CTTGCTCACAAACAAAACACCTTTCAGTGAGATACCTAGCATTTCTATTCCCTATTTTGT
GAACCAGGTAAAAGTTATATACATATCAACAATTTTTACTTAATGGAGAAGATTTTAACA
TGATGAATCTTTTTTTTTCTTTCAGGGCAAGAGACCAAGCCTATCGAATATTCCTGATGA
GGTCGTCCCCATATTAGAATGTTGTTGGGCAGCGGACTCAAAGACTCGTCTCGAATTCAA
AGATATTACAATCTCACTGGAAAGCTTGCTAAAAAGATTCTGCTCAGAGAGAAGTAATAA
TGAGATAACGATCACCGAAGATGAAGCTTATGAGATATATTGCGGACGTTAACAAATTGA
CGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTT
TTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGC
AGC
   
Sequence Name 46-K107289-SALK_103601C-R210
GenBank Accession LN485408 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K107289-SALK_103601C-R210
GCGTCATTTGTTACGTCCGCATATATCTCATAAGCTTCATCTTCGGTGATCGTTATCTCT
TTATTACTTCTCTCTGAGCAGAATCTTTTTAGCAAGCTTTCCAGTGAGATTGTAATATCT
TTGAATTCGAGACGAGTCTTTGAGTCCGCTGCCCAACAACATTCTAATATGGGGACGACC
TCATCAGGAATATTCGATAGGCTTGGTCTCTTGCCCTGAAAGAAAAAAAAAGATTCATCA
TGTTAAAATCTTCTCCATTAAGTAAAAATTGTTGATATGTATATAACTTTTACCTGGTTC
ACAAAATAGGGAATAGAAATGCTAGGTATCTCACTGAAAGGTGTTTTGTTTGTGAGCAAG
GACCAGAAAATCAGTGCGAAGCTATACACATCGATTTTCTGGTCGTAGTGTTTTTTCTCC
CCAATTCGGAGTGGCTCACGGCTGCATACCTGTGGATACAAAACATACAATCATTAATCA
TGATATGTTCTAGTCAAATATTTAGTCTAGAAAGAATCATTACCTCTGGAGCCATCCAAC
GGTACGTGCCCGCCTCACAAGTCATGCCACCAAGAGTCTTTCTCCTTGCAA

Last Updated on 06.12.2018 16:11