Sequences from confirmation amplicon


Confirmation sequences for line SALK_103627c and hit At1g79170 are shown.
Line ID SALK_103627c
Locus At1g79170 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:29782441
Primers used for PCR 27ty - LBb3, show primer details
   
Sequence Name 86-K117830-SALK_103627C-27ty
GenBank Accession LN486299 [GenBank]
Sequencing primer gene specific primer, 27ty: 5'-ACGGACGTTTTCATAACACCTAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>86-K117830-SALK_103627C-27ty
ACACATACACAACACAGACGAATGTGTGAAAAAAATGACATTACTTTATCGGGATTGATT
CATTTGACGTCAAAAGTCACTAAATAATTCAGCAAAATAGAAATTTTATTGTTAAGGAAA
AAAAAAACAAATCAAAACTGGAGTAACAAGTTATGCATTGTTCATTAAAATTTATTTATA
TATAAAATTTTAGGATGATTTGCTGACCATGTTTGATAATCTATTTAAAAATATACCACC
TTAATATGCAAAACTAATTAAATAACACTAAAAAGGGATAAAAAGTCAAGTAACTCCCCG
TTAAAACATGAAAACACCCCCGTGAAAAGGAAAAAAAAAGTGACGTGATCTTTCACACTT
TTTTTGGTTTATGGACATACTTAAAAAAAAAAAAGTGTTGGCTACCTAAATGATACCGAA
AATGGTAATAGTAACAATGACAGTGCCAATGGTGATGGTGACAACAACAATACCGAACAA
ATCACATTGACCCTAAAACAACTTAATAACACATTGCGAACGTTTTTAATGTACGGGGGT
GGTTTTTCTTTTCACCAGTGAAACGGGCAACACCTGATTGCCCTTCACCGCCGGGCCCTA
AAAAAGTTGCACCAAGCGGTCCACCCTGGTTTGCCCCACCAGGCAAAAATCCGGTTTGAT
GGTGGTTCCA
   
Sequence Name 69-K117829-SALK_103627C-R210
GenBank Accession LN485988 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>69-K117829-SALK_103627C-R210
TTTGTCCGCTATTGTTGTCGTCACCATCACCATCGGCACTGTCATTGTTACTATTACCAT
CTTCGCTATCATTAAGCTAGCCAACACTTTTCTCTTTTTATGTATGTCCATAAACCAAAA
AAAGTGTGAAAGATCACGTCACTCTTCTTCTCCTTTTCACGCGCGTGTTCTCATGTTTTA
ACGAGGAGTTACTTGACTTTTTATCCCTCTTTAGTGTTATTTACTTAGTTTTGCATATTA
AGGTGCTATATTCTTAAATAGATTATCAAACATGGTCAGCAAATCATCCTAAAATCTTAT
ATATAAATAAATCTTAATGAACAATGCATAACTTGTTACTCCAGTTTTGATTTGTTTTTT
TTTTCCTTAACAATAAAATTTCTATTTTGCTGAATTATTTAGTGACTTTTGACGTCAAAT
GAATCAATCCCAATAAAGTAATGTCATTTTTTTCACACATTCGCCGGTGTTTGTGTATGT
GTGTGTATAAGTTAATAAAAGCTATAAAATGTTAGGTGTTATGAAAACTTCC

Last Updated on 06.12.2018 16:11