Sequences from confirmation amplicon


Confirmation sequences for line SALK_104250 and hit At1g05750 are shown.
Line ID SALK_104250
Locus At1g05750 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:1721742
Primers used for PCR 25gm - LBb3, show primer details
   
Sequence Name 27-K112129-SALK_104250C-25gm
GenBank Accession LN485061 [GenBank]
Sequencing primer gene specific primer, 25gm: 5'-TGATTTGGATTTACTTGTGCTGAG-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K112129-SALK_104250C-25gm
TCTTAACGGATCAACCGGATTCTATTTTACCCGGTCTTATGTCCAAAGTCGGGTTTAGTT
AGAGATAAAAGGAAAAAATCAATTTTAAAAAGTCACCATCTCAATCGAATGGGTCTCCTT
CCCGTCGTCGGAATTACTTCGCCGGCGCTCATCACCCACAAAAATCACGCCAATCCAAAG
ATACAGAGACATAATCAATCTACCTCCGAGACCACTGTTTCATGGACTTCTCGCATCAAT
CTCCTCACGCGCAATGGTCGATTAGCGGAGGCAGCGAAGGAATTCTCCGATATGACACTC
GCCGGCGTAGAGCCTAACCACATCACTTTCATTGCAAATTGACGCTNAGACAACTTAATA
ACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGC
AACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTG
GTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCGA
   
Sequence Name 11-K112128-SALK_104250C-R210
GenBank Accession LN484584 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K112128-SALK_104250C-R210
CATTTGCATGAAGTGATGTGGTTAGGCTCTACGCCGGCGAGTGTCATATCGGAGAATTCC
TTCGCTGCCTCCGCTAATCGACCATTGCGCGTGAGGAGATTGATGCGAGAAGTCCATGAA
ACAGTGGTCTCGGAGGTAGATTGATTATGTCTCTGTATCTTTGGATTGGCGTGATTTTTG
TGGGTGATGAGCGCCGGCGAAGTAATTCCGACGACGGGAAGGAGACCCATTCGATTGAGA
TGGTGACTTTTTAAAATTGATTTTTTCCTTTTATCTCTAACTAAACCCGACTTTGGACAT
AAGACCGGGTAAAATAGAATCCGGTTTGATCCGGTTTTAAGATTTTAATTAAACGATAGT
CTATCTCAGCACAAGTAATTCCCAAATC

Last Updated on 06.12.2018 16:11