Sequences from confirmation amplicon


Confirmation sequences for line SALK_104777c and hit At4g22680 are shown.
Line ID SALK_104777c
Locus At4g22680 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:11923491
Primers used for PCR 16ck - LBb3, show primer details
   
Sequence Name 30-K108444-SALK_104777C-16ck
GenBank Accession LN485123 [GenBank]
Sequencing primer gene specific primer, 16ck: 5'-TAACTATCTCCGGCCTGACTTAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K108444-SALK_104777C-16ck
GACACTTGTCTAGATCTTCATGCTAATCTCGGCAATAAGTAAGCCTAATAACCATATATT
AATTCCACATTTTTTTTGAATTCTTACTTAATGATTTGTGTCATATAATATCAACGGAGG
CTTAAAATTTTCAAATTTATATTAAAAATTCAAATTTTACGTTGTAACAAAAACTAGAGA
CTTAAGATAAACATTGGTTTGATCATCATAAAATATAAAGTCAATTGCATGTGATCCAAC
ATGTTTAGTGTATTAATGTGTGTTGTGTAAATTATGCCAAATGGTTCCAAATAAGTTGGT
CAAATAAGTAGAATAAGTTTTGATTTGCATTGATTTTTCGTTGAAATCAACTTCAAGCTA
CTTCAGGATATATTGTGGTGTAAACAAATTGACGCTTAGACAACTTAATAACACATTGCG
GACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCATTGAGACGGGCACCAGCTGAT
TGCC
   
Sequence Name 14-K108443-SALK_104777C-R210
GenBank Accession LN484660 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K108443-SALK_104777C-R210
CATTTGTTACCCACAATATATCCTGAAGTAGCTTGAAGTTGATTTCAACGACAAATCAAT
GCAAATCAAAACTTATTCTACTTATTTGACCAACTTATTTGGAACCATTTGGCATAATTT
ACACAACACACATTAATACACTAAACATGTTGGATCACATGCAATTGACTTTATATTTTA
TGATGATCAAACCAATGTTTATCTTAAGTCTCTAGTTTTTGTTACAACGTAAAATTTGAA
TTTTTAATATAAATTTGAAAATTTTAAGCCTCCGTTGATATTATATGACACAAATCATTA
AGTAAGAATTCAAAAAAAATGTGGAATTAATATATGATTATTAGGCTTACTTATTGCCGA
GATTAGCATGAAGATCTATGACAAGTTGTTCTTCATCATGCGAGAGAAGGCCTCGTTTTA
AGTCAGGCCGGAGATAGTTA

Last Updated on 06.12.2018 16:11