Sequences from confirmation amplicon


Confirmation sequences for line SALK_106046c and hit At5g09740 are shown.
Line ID SALK_106046c
Locus At5g09740 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:3023905
Primers used for PCR 27eb - LBb3, show primer details
   
Sequence Name 50-K110195-SALK_106046C-27eb
GenBank Accession LN485504 [GenBank]
Sequencing primer gene specific primer, 27eb: 5'-TGGGTACTATCTTCAGCAATGAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K110195-SALK_106046C-27eb
TGTGAACCAGACTATTGAAGCTCGCAATGGAAATGTAATTAAAAAGCATCATAAGACTTC
AAAGATAAAGAAAAACAGTTGCTCACCATATGCCTTTGAAGCTGCTCTTTGCGTTTCATG
AAGTTCAGGCAAAACTCACAAAAAAAGAGCTTCACACAGTCATTGTATTCTGGCGGAAAA
GGGGAGAAGTACCAAGTCTCAATCTCATATTTTCCAAGCTCAATTGTTGATATGTTCTTC
ACTTTCGTGAACTCTTCATGTTCACGCAAACTTGCTGCATCCAGCTCTTCATGACCCTAT
AAAAACATTTCGAAACACAGAATACTGAATCAACACAAACCTTACGTTTCCAGGACCCGC
CTTATATTGGCATTCATTACCTCTATATGTGTCTCATCGATCTTCCTCTTCTGGTGACGT
GTCATCTTCAAGCTTGTTACCTAGAAACCACAGACATAAATCTCTGCTCATTGCGGACGT
ATTGACTGGGGTGTAAACAAATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTT
TTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTT
CACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCG
AAAATCCTGTTTGATGGTGGTTCCGAA
   
Sequence Name 42-K110194-SALK_106046C-R210
GenBank Accession LN485328 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K110194-SALK_106046C-R210
GCGTCATTTGTTTCACCCCAGTCAATACGTCCGCAATGAGCAGAGATTTATGTCTGTGGT
TTCTAGGTAACAAGCTTGAAGATGACACGTCACCAGAAGAGGAAGATCGATGAGACACAT
ATAGAGGTAATGAATGCCAATATAAGGCGGGTCCTGGAAACGTAAGGTTTGTGTTGATTC
AGTATTCTGTGTTTCGAAATGTTTTTATAGGGTCATGAAGAGCTGGATGCAGCAAGTTTG
CGTGAACATGAAGAGTTCACGAAAGTGAAGAACATATCAACAATTGAGCTTGGAAAATAT
GAGATTGAGACTTGGTACTTCTCCCCTTTTCCGCCAGAATACAATGACTGTGTGAAGCTC
TTTTTTTGTGAGTTTTGCCTGAACTTCATGAAACGCAAAGAGCAGCTTCAAAGGCATATG
GTGAGCAACTGTTTTTCTTTATCTTTGAAGTCTTATGATGCTTTTTAATTACATTTCCAT
TGCGAGCTTCAATAGTCTGGTTTCACATCTGTATTCTATGTTTTTGCATTCCAGTTTCAT
TGCTGAAGATAGTACCC
Primers used for PCR CE74 - LBb3, show primer details
   
Sequence Name 21-K119050-SALK_106046C-CE74
GenBank Accession LN484901 [GenBank]
Sequencing primer gene specific primer, CE74: 5'-AAAGGTGGAAATTTAGTCAAGGGT-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K119050-SALK_106046C-CE74
ATTGCAGACTTTATCAATTGGATCATTGGAATTGGCTTCAAGTTGTGTGAGATTTTTGAG
AAGGAGATCAAATTCTGCATTATACTTTGCTTCTAAAGATTTTGAACCATTTATACTATT
GTGATTAAGCTGAGTAATATGATGACAAAGTTGGTGATTTTGAAATCTTATGATCATCAA
TGTGTTTGATTTTGGTAATTTGTTTTCGAAACTGCAGTTAATAGGAGGCTGGATGAATGG
ACTCAGCTGGACCAACTGGACCTTGATTCAGTAGAGTGCGCTGTAGATGAAAAAGTGGAA
GACAAGGTGCGTTCTCTAGTTTTGTATTGTTAGTTGCATAATGTGGATTTCATGAGTTTT
CTCATATACGTTTAGACAAATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTT
TAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTC
ACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGA
AAATCCTGTTTGATGGTGGTTCCGAAATAAGCAAAA
   
Sequence Name 05-K119049-SALK_106046C-R210
GenBank Accession LN484417 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K119049-SALK_106046C-R210
TAACGTATATGAGAAAACTCATGAAATCCACATTATGCAACTAACAATACAAAACTAGAG
AACGCACCTTGTCTTCCACTTTTTCATCTACAGCGCACTCTACTGAATCAAGGTCCAGTT
GGTCCAGCTGAGTCCATTCATCCAGCCTCCTATTAACTGCAGTTTCGAAAACAAATTACC
AAAATCAAACACATTGATGATCATAAGATTTCAAAATCACCAACTTTGTCATCATATTAC
TCAGCTTAATCACAATAGTATAAATGGTTCAAAATCTTTAGAAGCAAAGTATAATGCAGA
ATTTGATCTCCTTCTCAAAAATCTCACACAACTTGAAGCCAATTCCAATGATCCAATTGA
TAAAGTCTTGCAATTTAACTCTTTTTTCTTCCTACATTATAAACCCTTGACTAAATTTCC
ACCTTT

Last Updated on 06.12.2018 16:11