Sequences from confirmation amplicon


Confirmation sequences for line SALK_106192 and hit At2g32380 are shown.
Line ID SALK_106192
Locus At2g32380 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:13746939
Primers used for PCR 32jd - LBb3, show primer details
   
Sequence Name 30-K113259-SALK_106192C-32jd
GenBank Accession LN485131 [GenBank]
Sequencing primer gene specific primer, 32jd: 5'-AATCTCGCTGTTTCTGATCAATTT-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K113259-SALK_106192C-32jd
GATTTTGTTGATTATGGATCTGAAATCTGAGATTAATGAAGATTATAGGAGAAATGATTA
TTGGTGATCAATGTTAAAGTCTGTGAAACTGGACCATTACAAAGTATGAACTAGGAGCAG
ACTAGTGAAATTTGTATAATGCTTGATTCAAGATTTCATTTGGTTCCCTAAAATCACAAC
ATTGTATGTTGATGAGGGAGAGAGATTTTGAGTTTCTACTATGTTGAATGACTAGTTTCA
TGGTTATTAGAATTAGTAACGTTATCGAACTTATACTTATTGTTCTGCAATGTGAATCTT
TCCGCATTCATTGCTAATATGCCTTGTGATGAAGAATCTGATATTGAGTATTCATTACTT
ATTAGGCTGCTATACTGGGAGAGATGATAGGATCAGGGAAGGCATCTGAGACGCTTAGAC
AACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGT
GAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGG
TCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCGAATCGGGCA
AAA
   
Sequence Name 13-K113258w-SALK_106192C-R210
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K113258w-SALK_106192C-R210
CTTCCCTGATCCTATCATCTCTCCCAGTATAGCAGCCTAATAAGTAATGAATACTCAATA
TCAGATTCTTCATCACAAGGCATATTAGCAATGAATGCGGAAAGATTCACATTGCAGAAC
AATAAGTATAAGTTCGATAACGTTACTAATTCTAATAACCATGAAACTAGTCATTCAACA
TAGTAGAAACTCAAAATCTCTCTCCCTCATCAACATACAATGTTGTGATTTTAGGGAACC
AAATGAAATCTTGAATCAAGCATTATACAAATTTCACTAGTCTGCTCCTAGTTCATACTT
TGTAATGGTCCAGTTTCACAGACTTTAACATTGATCACCAATAATCATTTCTCCTATAAT
CTTCATTAATCTCAGATTTCAGATCCATAAATCAACAAAATCTTACTCTAAATCAAACAC
GTTATATCCAAATTGATCAGA

Last Updated on 06.12.2018 16:11